Wang Zhe, Huang Jianbang, Chen Qimeng, Yu Yuanhua, Yu Xuan, Zhao Yue, Wang Yan, Shi Chunxiang, Zhao Zizhao, Tang Dachun
School of Life Science and Technology, Changchun University of Science and Technology, Changchun 130022, China.
School of Optoelectronics Engineering, Changchun University of Science and Technology, Changchun 130022, China.
Micromachines (Basel). 2025 Jul 2;16(7):789. doi: 10.3390/mi16070789.
To tackle the low-accuracy problem with analyzing urine component concentrations in real time, a fully automated dipstick analysis device of urine dry chemistry was designed, and a prediction method combining an image acquisition system with a whale optimization algorithm (WOA) for BP neural network optimization was proposed. The image acquisition system, which comprised an ESP32S3 chip and a GC2145 camera, was used to collect the urine test strip images, and then color data were calibrated by image processing and color correction on the upper computer. The correlations between reflected light and concentrations were established following the Kubelka-Munk theory and the Beer-Lambert law. A mathematical model of urine colorimetric value and concentration was constructed based on the least squares method. The WOA algorithm was applied to optimize the weight and threshold of the BP neural network, and substantial data were utilized to train the neural network and perform comparative analysis. The experimental results show that the MAE, RMSE and R of predicted versus actual urine protein values were, respectively, 3.1415, 4.328 and approximately 1. The WOA-BP neural network model exhibited high precision and accuracy in predicting the urine component concentrations.
为解决实时分析尿液成分浓度时准确性较低的问题,设计了一种全自动尿液干化学试纸分析装置,并提出了一种将图像采集系统与用于优化BP神经网络的鲸鱼优化算法(WOA)相结合的预测方法。该图像采集系统由ESP32S3芯片和GC2145相机组成,用于采集尿液试纸图像,然后在上位机上通过图像处理和色彩校正对颜色数据进行校准。根据库贝尔卡-蒙克理论和比尔-朗伯定律建立反射光与浓度之间的相关性。基于最小二乘法构建尿液比色值与浓度的数学模型。应用WOA算法优化BP神经网络的权重和阈值,并利用大量数据训练神经网络并进行对比分析。实验结果表明,预测的尿液蛋白质值与实际值的平均绝对误差(MAE)、均方根误差(RMSE)和相关系数(R)分别为3.1415、4.328和约1。WOA-BP神经网络模型在预测尿液成分浓度方面表现出高精度和准确性。