Ben Halima Hamdi, Bellagambi Francesca G, Hangouët Marie, Alcacer Albert, Pfeiffer Norman, Heuberger Albert, Zine Nadia, Bausells Joan, Elaissari Abdelhamid, Errachid Abdelhamid
Institute of Analytical Sciences (ISA) - UMR 5280, Claude Bernard Lyon 1 University (UCBL), 69100, Lyon, France.
Institute of Analytical Sciences (ISA) - UMR 5280, Claude Bernard Lyon 1 University (UCBL), 69100, Lyon, France.
Talanta. 2023 Jan 1;251:123759. doi: 10.1016/j.talanta.2022.123759. Epub 2022 Aug 5.
Heart failure (HF) is a chronic cardiovascular disease that represents main cause of mortality worldwide, particularly for elderly. N-terminal pro-brain natriuretic peptide (NT-proBNP) was identified as the gold standard biomarker for HF diagnosis and therapy monitoring. Presently, saliva analysis represents an emerging and powerful tool for clinical applications and electrochemical immunosensors have shown their potential in Healthcare applications as selective and reliable systems for detecting clinical biomarkers. This work presents the detection of NT-proBNP in saliva samples by an immunologically modified Field effect Transistor (IMFET). TESUD ((11-triethoxysilyl) undecanal) was used as cross-linker to immobilise anti-NT-proBNP antibody onto the device. Our IMFET that was then tested in different matrices (e.g. phosphate buffered saline (PBS), artificial saliva and human saliva) using electrochemical impedance spectroscopy (EIS), and it resulted selective to NT-proBNP with good sensitivity (detection limit of 0.02 pg/mL) and a wide linear range (0.02-1 pg/mL and 0.5-20 pg/mL). Finally, NT-proBNP concentration in ten saliva samples was determined by performing the standard addition method. An enzyme-linked immunosorbent assay was used for confirming IMFET results, highlighting both IMFET accuracy (analyte recovery of 99 ± 8%) and precision (coefficient of variation always <10%), and supporting the suitability of the device for determining salivary NT-proBNP.
心力衰竭（HF）是一种慢性心血管疾病，是全球范围内主要的死亡原因，尤其是在老年人中。N端前脑钠肽（NT-proBNP）被确定为心力衰竭诊断和治疗监测的金标准生物标志物。目前，唾液分析是临床应用中一种新兴且强大的工具，电化学免疫传感器已在医疗保健应用中显示出其作为检测临床生物标志物的选择性和可靠系统的潜力。这项工作展示了通过免疫修饰的场效应晶体管（IMFET）检测唾液样本中的NT-proBNP。TESUD（（11-三乙氧基甲硅烷基）十一醛）用作交联剂，将抗NT-proBNP抗体固定在该装置上。然后使用电化学阻抗谱（EIS）在不同基质（如磷酸盐缓冲盐水（PBS）、人工唾液和人类唾液）中对我们的IMFET进行测试，结果表明它对NT-proBNP具有选择性，灵敏度良好（检测限为0.02 pg/mL）且线性范围宽（0.02 - 1 pg/mL和0.5 - 20 pg/mL）。最后，通过标准加入法测定了十个唾液样本中的NT-proBNP浓度。使用酶联免疫吸附测定法来确认IMFET的结果，突出了IMFET的准确性（分析物回收率为99±8%）和精密度（变异系数始终<10%），并支持该装置用于测定唾液中NT-proBNP的适用性。
