Lam Johnny, Kim Kyobum, Lu Steven, Tabata Yasuhiko, Scott David W, Mikos Antonios G, Kasper F Kurtis
Department of Bioengineering, Rice University, Houston, Texas.
J Biomed Mater Res A. 2014 Oct;102(10):3477-87. doi: 10.1002/jbm.a.35015. Epub 2013 Nov 15.
In this study, a full factorial approach was used to investigate the effects of poly(ethylene glycol) (PEG) molecular weight (MW; 10,000 vs. 35,000 nominal MW), crosslinker-to-macromer carbon-carbon double bond ratio (DBR; 40 vs. 60), crosslinker type (PEG-diacrylate (PEGDA) vs. N,N'-methylene bisacrylamide (MB)), crosslinking extent of incorporated gelatin microparticles (low vs. high), and incubation medium composition (with or without collagenase) on the swelling and degradation characteristics of oligo[(poly(ethylene glycol) fumarate)] (OPF) hydrogel composites as indicated by the swelling ratio and the percentage of mass remaining, respectively. Each factor consisted of two levels, which were selected based on previous in vitro and in vivo studies utilizing these hydrogels for various tissue engineering applications. Fractional factorial analyses of the main effects indicated that the mean swelling ratio and the mean percentage of mass remaining of OPF composite hydrogels were significantly affected by every factor. In particular, increasing the PEG chain MW of OPF macromers significantly increased the mean swelling ratio and decreased the mean percentage of mass remaining by 5.7 ± 0.3 and 17.2 ± 0.6%, respectively. However, changing the crosslinker from MB to PEGDA reduced the mean swelling ratio and increased the mean percentage of mass remaining of OPF composite hydrogels by 4.9 ± 0.2 and 9.4 ± 0.9%, respectively. Additionally, it was found that the swelling characteristics of hydrogels fabricated with higher PEG chain MW or with MB were more sensitive to increases in DBR. Collectively, the main and cross effects observed between factors enables informed tuning of the swelling and degradation properties of OPF-based hydrogels for various tissue engineering applications. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 3477-3487, 2014.
在本研究中，采用全因子试验设计方法，研究聚乙二醇（PEG）分子量（MW；标称分子量10,000与35,000）、交联剂与大分子单体碳 - 碳双键比（DBR；40与60）、交联剂类型（聚乙二醇二丙烯酸酯（PEGDA）与N,N'-亚甲基双丙烯酰胺（MB））、掺入的明胶微粒交联程度（低与高）以及孵育介质组成（含或不含胶原酶）对聚（富马酸乙二醇酯）（OPF）水凝胶复合材料溶胀和降解特性的影响，分别用溶胀率和剩余质量百分比表示。每个因素包含两个水平，这些水平是根据先前利用这些水凝胶用于各种组织工程应用的体外和体内研究选定的。主效应的部分因子分析表明，OPF复合水凝胶的平均溶胀率和平均剩余质量百分比均受到每个因素的显著影响。特别地，增加OPF大分子单体的PEG链MW可显著提高平均溶胀率，并使平均剩余质量百分比分别降低5.7±0.3%和17.2±0.6%。然而，将交联剂从MB更换为PEGDA可使OPF复合水凝胶的平均溶胀率降低4.9±0.2%，并使平均剩余质量百分比增加9.4±0.9%。此外，发现用较高PEG链MW或MB制备的水凝胶的溶胀特性对DBR的增加更为敏感。总体而言，各因素之间观察到的主效应和交叉效应有助于针对各种组织工程应用，对基于OPF的水凝胶的溶胀和降解特性进行明智的调控。©2013威利期刊公司。《生物医学材料研究杂志》A部分：102A：3477 - 3487，2014年。
