Fakfum Puriwat, Chuljerm Hataichanok, Parklak Wason, Roytrakul Sittiruk, Phaonakrop Narumon, Lerttrakarnnon Peerasak, Kulprachakarn Kanokwan
Research Institute for Health Sciences, Chiang Mai University, Chiang Mai 50200, Thailand.
National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, Pathum Thani 12120, Thailand.
Life (Basel). 2024 Oct 5;14(10):1269. doi: 10.3390/life14101269.
The study explored proteomics to better understand the relationship between type 2 diabetes (T2DM) and hypertension (HT) in Thai adults, using shotgun proteomics and bioinformatics analysis. Plasma samples were taken from 61 subjects: 14 healthy subjects (mean age = 40.85 ± 7.12), 13 with T2DM (mean age = 57.38 ± 6.03), 16 with HT (mean age = 66.87 ± 10.09), and 18 with coexisting T2DM/HT (mean age = 58.22 ± 10.65). Proteins were identified using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Protein-protein interactions were analyzed using the Search Tool for the Retrieval of Interacting Genes/Proteins (STRING) version 11.5. We identified six unique proteins in T2DM patients, including translationally controlled 1 (TPT1) and nibrin (NBN), which are associated with the DNA damage response. In HT patients, seven unique proteins were identified, among them long-chain fatty acid-CoA ligase (ASCL), which functions in the stimulation of triacylglycerol and cholesterol synthesis, and NADPH oxidase activator 1 (NOXA1), which is involved in high blood pressure via angiotensin II-induced reactive oxygen species (ROS)-generating systems. In coexisting T2DM/HT patients, six unique proteins were identified, of which two-microtubule-associated protein 1A (MAP1A)-might be involved in dementia via RhoB-p53 and diacylglycerol kinase beta (DGKB), associated with lipid metabolism. This study identified new candidate proteins that are possibly involved in the pathology of these diseases.
该研究采用鸟枪法蛋白质组学和生物信息学分析，探索蛋白质组学以更好地理解泰国成年人2型糖尿病（T2DM）与高血压（HT）之间的关系。从61名受试者采集血浆样本：14名健康受试者（平均年龄 = 40.85 ± 7.12）、13名T2DM患者（平均年龄 = 57.38 ± 6.03）、16名HT患者（平均年龄 = 66.87 ± 10.09）以及18名T2DM/HT共存患者（平均年龄 = 58.22 ± 10.65）。使用液相色谱 - 串联质谱（LC-MS/MS）鉴定蛋白质。使用基因/蛋白质相互作用检索搜索工具（STRING）11.5版分析蛋白质 - 蛋白质相互作用。我们在T2DM患者中鉴定出六种独特蛋白质，包括与DNA损伤反应相关的翻译控制肿瘤蛋白1（TPT1）和尼布林（NBN）。在HT患者中，鉴定出七种独特蛋白质，其中长链脂肪酸辅酶A连接酶（ASCL）在三酰甘油和胆固醇合成刺激中起作用，以及NADPH氧化酶激活剂1（NOXA1），其通过血管紧张素II诱导的活性氧（ROS）生成系统参与高血压。在T2DM/HT共存患者中，鉴定出六种独特蛋白质，其中两种——微管相关蛋白1A（MAP1A）——可能通过RhoB - p53参与痴呆，以及二酰甘油激酶β（DGKB），与脂质代谢相关。本研究鉴定出可能参与这些疾病病理过程的新候选蛋白质。
