Translational Gerontology Branch, National Institute on Aging, NIH, Baltimore, MD, 21224, USA.
National Institute on Aging, Intramural Research Program, Clinical Research Core, NIH, Baltimore, MD, 21224, USA.
Geroscience. 2021 Apr;43(2):1029-1037. doi: 10.1007/s11357-020-00288-9. Epub 2020 Nov 17.
Frailty is a risk factor for poor health outcomes in older adults. The aim of this study was to identify plasma proteomic biomarkers of frailty in 752 men and women older than 65 years of age from the InCHIANTI study. One thousand three hundred one plasma proteins were measured using an aptamer-based assay. Associations of each protein with frailty status were assessed using logistic regression and four proteins creatine kinase M-type (CKM), B-type (CKB), C-X-C motif chemokine ligand 13 (CXCL13), and thrombospondin 2 (THBS2) were associated with frailty status. Two proteins, cyclin-dependent kinase 5 (CDK5/CDK5R1) and interleukin 1 alpha (IL1A), were associated with worsening of frailty status over time in volunteers free of frailty at baseline. Using partial least squares discriminant analysis (PLS-DA), data of 1301 proteins was able to discriminate between frail and non-frail with a 2% error rate. The proteins with greater discriminatory ability represented the inflammation, blood coagulation, and cell growth pathways. The utility of these proteins as biomarkers of frailty should be further explored.
衰弱是老年人健康状况不佳的一个风险因素。本研究旨在确定 752 名年龄在 65 岁以上的 InCHIANTI 研究中的男性和女性血浆蛋白质组学衰弱生物标志物。使用基于适体的测定法测量了 1301 种血浆蛋白。使用逻辑回归评估每种蛋白质与衰弱状态的关联，并且有 4 种蛋白质肌酸激酶 M 型（CKM）、B 型（CKB）、C-X-C 基序趋化因子配体 13（CXCL13）和血小板反应蛋白 2（THBS2）与衰弱状态相关。两种蛋白质，周期蛋白依赖性激酶 5（CDK5/CDK5R1）和白细胞介素 1α（IL1A），与基线时无衰弱志愿者的衰弱状态随时间恶化相关。使用偏最小二乘判别分析（PLS-DA），可以以 2%的错误率区分脆弱和非脆弱。具有更高区分能力的蛋白质代表炎症、血液凝固和细胞生长途径。应该进一步探索这些蛋白质作为衰弱生物标志物的效用。
