Ermakov Evgeny A, Melamud Mark M, Boiko Anastasiia S, Ivanova Svetlana A, Sizikov Alexey E, Nevinsky Georgy A, Buneva Valentina N
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, Russia.
Department of Natural Sciences, Novosibirsk State University, 630090 Novosibirsk, Russia.
Life (Basel). 2024 Oct 14;14(10):1305. doi: 10.3390/life14101305.
Patients with systemic lupus erythematosus (SLE) are known to frequently suffer from comorbid cardiovascular diseases (CVDs). There are abundant data on cytokine levels and their role in the pathogenesis of SLE, while growth factors have received much less attention. The aim of this study was to analyze growth factor levels in SLE patients and their association with the presence of comorbid CVDs. The serum concentrations for the granulocyte-macrophage colony-stimulating factor (GM-CSF), nerve growth factor β (NGFβ), glial cell line-derived neurotrophic factor (GDNF), and neuregulin-1 β (NRG-1β) were determined in the SLE patients (n = 35) and healthy individuals (n = 38) by a Luminex multiplex assay. The NGFβ and NRG-1β concentrations were shown to be significantly higher in the total group of SLE patients (median [Q1-Q3]: 3.6 [1.3-4.5] and 52.5 [8.5-148], respectively) compared with the healthy individuals (2.9 [1.3-3.4] and 13.7 [4.4-42] ng/mL, respectively). The GM-CSF and GDNF levels did not differ. Interestingly, elevated NRG-1β levels were associated with the presence of CVDs, as SLE patients with CVDs had significantly higher NRG-1β levels (99 [22-242]) compared with the controls (13.7 [4.4-42]) and patients without CVDs (19 [9-80] ng/mL). The model for the binary classification of SLE patients with and without CVDs based on the NRG-1β level had an average predictive ability (AUC = 0.67). Thus, altered levels of growth factors may be associated with comorbid CVDs in SLE patients.
已知系统性红斑狼疮（SLE）患者常并发心血管疾病（CVD）。关于细胞因子水平及其在SLE发病机制中的作用已有大量数据，而生长因子受到的关注则少得多。本研究的目的是分析SLE患者的生长因子水平及其与并发CVD的关系。通过Luminex多重检测法测定了35例SLE患者和38例健康个体血清中粒细胞-巨噬细胞集落刺激因子（GM-CSF）、神经生长因子β（NGFβ）、胶质细胞源性神经营养因子（GDNF）和神经调节蛋白-1β（NRG-1β）的浓度。结果显示，SLE患者组中NGFβ和NRG-1β的浓度（中位数[四分位数间距]：分别为3.6[1.3 - 4.5]和52.5[8.5 - 148]）显著高于健康个体（分别为2.9[1.3 - 3.4]和13.7[4.4 - 42] ng/mL）。GM-CSF和GDNF水平无差异。有趣的是，NRG-1β水平升高与CVD的存在相关，因为患有CVD的SLE患者的NRG-1β水平（99[22 - 242]）显著高于对照组（13.7[4.4 - 42]）和无CVD的患者（19[9 - 80] ng/mL）。基于NRG-1β水平对有或无CVD的SLE患者进行二元分类的模型具有中等预测能力（曲线下面积 = 0.67）。因此，生长因子水平的改变可能与SLE患者并发CVD有关。
