Yu Zhihao, Fu Xiali, Lucas Theotim, Zhao Heng, Chen Changchong, Dubail Iharilalao, Chen Yong, Patriarche Gilles, Gateau Jérôme, Gazeau Florence, Jamet Anne, Lepoitevin Mathilde, Serre Christian
Institut des Matériaux Poreux de Paris, ENS, ESPCI Paris, CNRS, PSL University, Paris, 75005, France.
INSERM UMR-S1151, CNRS UMR-S8253, Institut Necker Enfants Malades, Team « Pathogenesis of systemic infections », Université Paris Cité, Paris, F-75015, France.
Adv Healthc Mater. 2025 Jan;14(1):e2402418. doi: 10.1002/adhm.202402418. Epub 2024 Oct 26.
Phototherapy is a low-risk alternative to traditional antibiotics against drug-resistant bacterial infections. However, optimizing phototherapy agents, refining treatment conditions, and addressing misuse of agents, remain a formidable challenge. This study introduces a novel concept leveraging the unique customizability of metal-organic frameworks (MOFs) to house size-matched dye molecules in "single rooms". The mesoporous iron(III) carboxylate nanoMOF, MIL-100(Fe), and the hydrophobic heptamethine cyanine photothermal dye (Cy7), IR775, are selected as model systems. Their combination is predicted to minimize dye-dye interactions, leading to exceptional photostability and efficient light-to-heat conversion. Furthermore, MIL-100(Fe) preserves the antimicrobial nature of hydrophobic IR775, enabling it to disrupt bacterial cell envelopes. Through electrospinning, MIL-100(Fe)@IR775 nanoparticles are shaped into a gelatin-based film dressing for the treatment of skin wounds infected by Methicillin-resistant Staphylococcus aureus (MRSA). Activation of the dressing requires only a portable near-infrared light-emitting diode (NIR LED) and induces both low-dose photodynamic therapy (LPDT) and mild-temperature photothermal therapy (MPTT). Combined with the antimicrobial properties of IR775 and ferroptosis-like lipid peroxidation induced by MIL-100(Fe), the photoactive dressing eradicates MRSA and the healing is as quick as the uninfected wounds. This safe, cost-effective, and multifunctional therapeutic wound dressing offers a promising solution to overcome the current bottleneck in phototherapy.
光疗是一种针对耐药细菌感染的低风险替代传统抗生素的方法。然而，优化光疗剂、完善治疗条件以及解决药剂滥用问题，仍然是一项艰巨的挑战。本研究引入了一种新颖的概念，利用金属有机框架（MOF）独特的可定制性，将尺寸匹配的染料分子容纳在“单间”中。选择介孔羧酸铁（III）纳米MOF、MIL-100（Fe）和疏水性七甲川花菁光热染料（Cy7）、IR775作为模型系统。预计它们的组合可使染料-染料相互作用最小化，从而实现卓越的光稳定性和高效的光热转换。此外，MIL-100（Fe）保留了疏水性IR775的抗菌特性，使其能够破坏细菌细胞膜。通过静电纺丝，将MIL-100（Fe）@IR775纳米颗粒制成基于明胶的薄膜敷料，用于治疗耐甲氧西林金黄色葡萄球菌（MRSA）感染的皮肤伤口。该敷料的激活仅需一个便携式近红外发光二极管（NIR LED），并诱导低剂量光动力疗法（LPDT）和温和温度光热疗法（MPTT）。结合IR775的抗菌特性和MIL-100（Fe）诱导的铁死亡样脂质过氧化作用，这种光活性敷料可根除MRSA，且愈合速度与未感染伤口一样快。这种安全、经济高效且多功能的治疗性伤口敷料为克服当前光疗的瓶颈提供了一个有前景的解决方案。
