Glickman L T, Grieve R B, Schantz P M
Am J Med. 1986 Feb;80(2):161-4. doi: 10.1016/0002-9343(86)90003-3.
Four symptomatic and four asymptomatic patients with histologically confirmed zoonotic pulmonary dirofilariasis caused by Dirofilaria immitis (dog heartworm) were evaluated serologically. Five patients had diagnostic indirect hemagglutination titers to D. immitis and six had positive findings by enzyme-linked immunosorbent assay. Of the two patients that had nondiagnostic titers by both enzyme-linked immunosorbent assay and indirect hemagglutination, one had an encapsulated necrotic adult worm that appeared to have been dead for some time, and the serum specimen for the second patient had been obtained five months following surgical removal of the granuloma. These findings suggest good sensitivity for these serologic methods in active cases, but declining antibody titers and decreased sensitivity following worm death. In general, cross-reactivity of the enzyme-linked immunosorbent assay with serum from patients with other nonfilarial parasitic infections or neoplasms was not observed.
对4例有症状和4例无症状的经组织学确诊为由犬恶丝虫（犬心丝虫）引起的人畜共患性肺恶丝虫病患者进行了血清学评估。5例患者对犬恶丝虫的诊断性间接血凝滴度呈阳性，6例通过酶联免疫吸附测定有阳性结果。在2例酶联免疫吸附测定和间接血凝滴度均无诊断意义的患者中，1例有一个包囊化坏死成虫，似乎已死亡一段时间，第二例患者的血清标本是在肉芽肿手术切除5个月后获得的。这些发现表明这些血清学方法在活动病例中具有良好的敏感性，但在虫体死亡后抗体滴度下降且敏感性降低。一般来说，未观察到酶联免疫吸附测定与其他非丝虫寄生虫感染或肿瘤患者血清的交叉反应。
