抗犬恶丝虫22kD分子抗体在人类肺恶丝虫病诊断中的应用。

Utility of antibodies against a 22 kD molecule of Dirofilaria immitis in the diagnosis of human pulmonary dirofilariasis.

作者信息

Perera L, Pérez-Arellano J L, Cordero M, Simón F, Muro A

机构信息

Laboratorio de Parasitología, Facultad de Farmacia, Universidad de Salamanca, Spain.

出版信息

Trop Med Int Health. 1998 Feb;3(2):151-5. doi: 10.1046/j.1365-3156.1998.00209.x.

DOI:10.1046/j.1365-3156.1998.00209.x

PMID:9537278

Abstract

To assess the characteristics of an ELISA test for the diagnosis of human pulmonary dirofilariasis, we studied the sera of 24 subjects with other helmintoses and of 37 patients suffering from non-parasitic focal lung diseases, comparing them with negative and positive sera. ELISA and Western blot with complete somatic antigen and ELISA with protein Di22 (specifically recognized in cases of lung dirofilariasis) were performed. With ELISA SA the false positive rate was 25% in cases with other parasitoses and 30% in cases with focal lung diseases. ELISA Di22 decreases this positivity levels. Only 2 cases with visceral larva migrans (8.3%) and a case with lung nodules metastatic from renal adenocarcinoma (2.7%) were positive. ELISA Di22 therefore greatly decreases the false positive rate of ELISA SA.

摘要

为评估用于诊断人类肺丝虫病的酶联免疫吸附测定（ELISA）试验的特征，我们研究了24名患有其他蠕虫病的受试者以及37名患有非寄生虫性局灶性肺病患者的血清，并将其与阴性和阳性血清进行比较。采用完整虫体抗原进行ELISA和蛋白质印迹分析，以及采用蛋白质Di22（在肺丝虫病病例中可特异性识别）进行ELISA检测。采用ELISA SA时，其他寄生虫病病例的假阳性率为25%，局灶性肺病病例的假阳性率为30%。ELISA Di22降低了这种阳性水平。仅2例内脏幼虫移行症患者（8.3%）和1例肾腺癌肺转移瘤患者（2.7%）呈阳性。因此，ELISA Di22大大降低了ELISA SA的假阳性率。