Perera L, Muro A, Cordero M, Villar E, Simón F
Laboratorio de Parasitología, Facultad de Farmacia, Universidad de Salamanca, Spain.
Trop Med Parasitol. 1994 Sep;45(3):249-52.
Human pulmonary dirofilariosis usually appears as a solitary pulmonary nodule. A 22 kDa antigen from Dirofilaria immitis adult worms specifically recognized by three sera from individuals diagnosed with pulmonary dirofilariosis was identified by enzyme-linked-immunoelectrotransfer blot. This antigen was purified by elution from acrylamide gels and evaluated in enzyme-linked immunoassay using sera from 92 patients with pulmonary dirofilariosis and other pulmonary and parasitic diseases. Its use in the diagnosis of human pulmonary dirofilariosis should be considered.
人类肺丝虫病通常表现为单个肺结节。通过酶联免疫电转移印迹法鉴定出一种来自犬恶丝虫成虫的22 kDa抗原，该抗原能被三名诊断为肺丝虫病患者的血清特异性识别。通过从丙烯酰胺凝胶上洗脱对该抗原进行纯化，并使用92例肺丝虫病患者以及其他肺部和寄生虫疾病患者的血清进行酶联免疫测定评估。应考虑将其用于人类肺丝虫病的诊断。
