Department of Preventive Medicine, Public Health and Microbiology, Universidad Autónoma de Madrid, Madrid, Spain.
Department of Pathology, Molecular, and Cell-Based Medicine, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
J Med Virol. 2024 Nov;96(11):e70023. doi: 10.1002/jmv.70023.
The 2022-2024 outbreak of MPOX is an important worldwide public health issue that has triggered significant concerns in the scientific community. MPOX is caused by monkeypox virus (MPXV) belonging to the Poxviridae family. The study of MPXV presents a multifaceted challenge due to the diverse viral formThis study was supported by ISIDORe consortium and Agencia Estatal de Investigación.s produced by this pathogen. Notably the intracellular mature viruses (MVs) primarily contribute to localized lesions and host-to-host transmission, while the extracellular enveloped viruses (EVs) are associated with systemic infection. Clinically, MPOX manifests as a vesiculopustular rash that initially emerges on the face and trunk, subsequently spreading throughout the body, with heightened severity observed in immunocompromised individuals. Results obtained in this manuscript indicate that the 2022 outbreak MPXV has a significantly slower viral cycle compared with previous Clade II strains, with WRAIR 7-61 being more intermediate and USA 2003 producing highest viral titers. Additionally, proteomic and phospho-proteomic analysis displays differences in protein expression between these three strains. These findings highlight key differences between the current Lineage B.1 MPXV and previous strains. Further studies will be undertaken to demonstrate if these differences are important for the apparent increased human-to-human transmission mechanisms observed in the Clade IIb MPXV outbreak.
2022-2024 年猴痘疫情是一个重要的全球公共卫生问题，引起了科学界的高度关注。猴痘是由正痘病毒科的猴痘病毒（MPXV）引起的。由于该病原体产生的多种病毒形式，MPXV 的研究具有多方面的挑战。本研究得到了 ISIDORe 联盟和西班牙国家研究委员会的支持。值得注意的是，细胞内成熟病毒（MVs）主要导致局部损伤和宿主间传播，而细胞外包膜病毒（EVs）与全身感染有关。临床上，猴痘表现为水疱脓疱疹，最初出现在面部和躯干，随后全身扩散，免疫功能低下者病情更为严重。本手稿中的结果表明，与以前的 II 型毒株相比，2022 年暴发的猴痘病毒的病毒周期明显较慢，WRAIR 7-61 更为中间，而 USA 2003 产生的病毒滴度最高。此外，蛋白质组学和磷酸化蛋白质组学分析显示这三种菌株之间的蛋白质表达存在差异。这些发现突出了当前 B.1 谱系 MPXV 与以前菌株之间的关键差异。将进一步开展研究，以证明这些差异是否对 IIb 型 MPXV 暴发中观察到的明显增加的人际传播机制很重要。
