Department of Science Education, Korea National University of Education, Cheongju-si, Republic of Korea.
Department of Hematology and Medical Oncology, Winship Cancer Institute of Emory, Emory University School of Medicine, Atlanta, GA, U.S.A.
Cancer Genomics Proteomics. 2024 Nov-Dec;21(6):608-621. doi: 10.21873/cgp.20477.
BACKGROUND/AIM: Glioma, the most common type of primary brain tumor, is characterized by high malignancy, recurrence, and mortality. Long non-coding RNA (lncRNA) H19 is a potential biomarker for glioma diagnosis and treatment due to its overexpression in human glioma tissues and its involvement in cell division and metastasis regulation. This study aimed to identify potential therapeutic targets involved in glioma development by analyzing gene expression profiles regulated by H19.
To elucidate the role of H19 in A172 and U87MG glioma cell lines, cell counting, colony formation, and wound healing assays were conducted. RNA-seq data analysis and bioinformatics analyses were performed to reveal the molecular interactions of H19.
Cell-based experiments showed that elevated H19 levels were related to cancer cell survival, proliferation, and migration. Bioinformatics analyses identified 2,084 differentially expressed genes (DEGs) influenced by H19 which are involved in cancer progression. Specifically, ANXA5, CLEC18B, RAB42, CXCL8, OASL, USP18, and CDCP1 were positively correlated with H19 expression, while CSDC2 and FOXO4 were negatively correlated. These DEGs were predicted to function as oncogenes or tumor suppressors in gliomas, in association with H19.
These findings highlight H19 and its associated genes as potential diagnostic and therapeutic targets for gliomas, emphasizing their clinical significance in patient survival.
背景/目的：脑胶质瘤是最常见的原发性脑肿瘤，其特点是恶性程度高、易复发、死亡率高。长链非编码 RNA（lncRNA）H19 由于在人胶质瘤组织中过度表达，并参与细胞分裂和转移调节，因此是胶质瘤诊断和治疗的潜在生物标志物。本研究旨在通过分析受 H19 调控的基因表达谱，鉴定与胶质瘤发生相关的潜在治疗靶点。
为了阐明 H19 在 A172 和 U87MG 神经胶质瘤细胞系中的作用，进行了细胞计数、集落形成和划痕愈合实验。进行 RNA-seq 数据分析和生物信息学分析，以揭示 H19 的分子相互作用。
基于细胞的实验表明，H19 水平升高与癌细胞的存活、增殖和迁移有关。生物信息学分析鉴定出 2084 个受 H19 影响的差异表达基因（DEGs），这些基因参与癌症进展。具体而言，ANXA5、CLEC18B、RAB42、CXCL8、OASL、USP18 和 CDCP1 与 H19 表达呈正相关，而 CSDC2 和 FOXO4 呈负相关。这些 DEGs 被预测为与 H19 相关的胶质瘤中的癌基因或抑癌基因，与 H19 相关。
这些发现强调了 H19 及其相关基因作为胶质瘤潜在诊断和治疗靶点的重要性，突出了它们在患者生存中的临床意义。
