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α-MSH 诱导的 B16F10 黑素瘤细胞中菌株后生物对 CREB/MITF 和 MAPKs 信号通路的抗黑色素生成和抗氧化作用。

Antimelanogenic and Antioxidant Effects of Postbioics of Strains in α-MSH-Induced B16F10 Melanoma Cells via CREB/MITF and MAPKs Signaling Pathway.

机构信息

Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul 05029, Republic of Korea.

出版信息

J Microbiol Biotechnol. 2024 Nov 28;34(11):2279-2289. doi: 10.4014/jmb.2408.08015. Epub 2024 Sep 30.

DOI:10.4014/jmb.2408.08015
PMID:39468979
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11637832/
Abstract

Abnormal melanin synthesis can lead to severe skin problems. This study investigated the anti-melanogenic effects on α-melanocyte stimulating hormone (α-MSH)-induced B16F10 cells using cell-free supernatants of WB326 and WB2810. Samples were prepared using 1 mg/ml freeze-dried culture supernatant. Cell viability was assessed using B16F10 cells and MTT assay. Tyrosinase inhibition and melanin content decreased in the samples compared to those treated with α-MSH. This effect was also observed when L-DOPA staining was used under a microscope. Moreover, the mRNA expression levels of microphthalmia-associated transcription factor (MITF), tyrosinase, tyrosinase-related protein (TRP)-1, and TRP-2 decreased in the sample-treated group. Protein expression of the CREB/MITF/MAPK signaling pathway was also reduced. Using HPLC analysis, lactic and acetic acids were detected in the culture supernatants. Finally, the antioxidant effects of the samples were confirmed by comparison with those of Trolox and arbutin. According to the experimental results, their utilization is possible in the fields of functional materials and cosmetic ingredients.

摘要

异常的黑色素合成会导致严重的皮肤问题。本研究使用 WB326 和 WB2810 的无细胞上清液研究了对 α-促黑素细胞激素 (α-MSH) 诱导的 B16F10 细胞的抗黑色素生成作用。样品采用 1mg/ml 冻干培养上清液制备。通过 MTT 测定法评估细胞活力。与 α-MSH 处理组相比，样品处理组的酪氨酸酶抑制率和黑色素含量降低。在显微镜下使用 L-DOPA 染色也观察到了这种效果。此外，样品处理组小眼畸形相关转录因子 (MITF)、酪氨酸酶、酪氨酸酶相关蛋白 (TRP)-1 和 TRP-2 的 mRNA 表达水平降低。CREB/MITF/MAPK 信号通路的蛋白表达也减少。通过 HPLC 分析，在培养上清液中检测到乳酸和乙酸。最后，通过与 Trolox 和熊果苷的比较，证实了样品的抗氧化作用。根据实验结果，它们可用于功能材料和化妆品成分领域。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2460/11637832/b7bc5490136a/jmb-34-11-2279-f7.jpg
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