胶体金 L. 提取物的抗氧化和抗黑色素生成作用。

Antioxidant and Anti-Melanogenesis Effects of Colloidal Gold L. Extracts.

机构信息

Department of Pharmaceutical & Cosmetics, Dongshin University, Gunjae-ro 185, Naju 58245, Jeonnam, Korea.

BOHYANG Tea Co., Ltd., 144, Dongam 1-gil, Boseong-eup, Boseong-gun 59462, Jeollanam-do, Korea.

出版信息

Molecules. 2022 Aug 30;27(17):5593. doi: 10.3390/molecules27175593.

DOI:10.3390/molecules27175593

PMID:36080359

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9457959/

Abstract

Green tea extract derived from the leaves of L. (CS), is a representative beverage with antioxidant, anti-cancer, and anti-viral properties. CS extract is also used in cosmetics. Colloidal gold is generally a sol or colloidal suspension of gold nanoparticles in water. Colloidal gold green tea (CGCS), cultivated as a fertilizer using this colloidal gold solution, contains gold minerals and possesses anti-inflammatory, analgesic, and anti-tumor properties. However, the skin bioactivity of CGCS has not yet been investigated. In this study, we investigated the effect of the CGCS extract on skin whitening. CGCS extract contained high levels of phenols and flavonoids and displayed 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity in a concentration-dependent manner. CGCS extract inhibited melanin synthesis and tyrosinase activity in B16F10 cells more effectively than the CS extract. Moreover, the CGCS extract decreased the expression levels of the melanogenesis-related proteins, tyrosinase, tyrosinase-related proteins (TRPs), and microphthalmia-associated transcription factor (MITF). In conclusion, our study showed that the CGCS extract inhibits the expression of tyrosinase, TRP-1, and TRP-2 via the downregulation of MITF, thereby inhibiting melanin synthesis. Therefore, CGCS can potentially be used as a skin-whitening ingredient in the cosmetic industry.

摘要

绿茶提取物来源于 L. 的叶子，是一种具有抗氧化、抗癌和抗病毒特性的代表性饮料。CS 提取物也用于化妆品。胶体金通常是金纳米粒子在水中的溶胶或胶体悬浮液。胶体金绿茶（CGCS）是用这种胶体金溶液作为肥料培养的，含有金矿物质，具有抗炎、镇痛和抗肿瘤的特性。然而，CGCS 的皮肤生物活性尚未得到研究。在这项研究中，我们研究了 CGCS 提取物对皮肤美白的影响。CGCS 提取物含有高水平的酚类化合物和类黄酮，并表现出 2,2-二苯基-1-苦基肼（DPPH）自由基清除活性，呈浓度依赖性。CGCS 提取物在 B16F10 细胞中比 CS 提取物更有效地抑制黑色素合成和酪氨酸酶活性。此外，CGCS 提取物降低了黑色素生成相关蛋白、酪氨酸酶、酪氨酸酶相关蛋白（TRP）和小眼畸形相关转录因子（MITF）的表达水平。总之，我们的研究表明，CGCS 提取物通过下调 MITF 抑制酪氨酸酶、TRP-1 和 TRP-2 的表达，从而抑制黑色素合成。因此，CGCS 有可能在化妆品行业用作皮肤美白成分。