A Iraqi National Cancer Research Center, University of Baghdad, Baghdad, Iraq.
Department of Science, College of Basic Education, Mustansiriyah University, Baghdad, Iraq.
Asian Pac J Cancer Prev. 2024 Oct 1;25(10):3651-3660. doi: 10.31557/APJCP.2024.25.10.3651.
The objective of this study is to analyze oxidative stress markers Total Oxidant Status (TOS) and total Antioxidant Status (TAS), assess antioxidant levels (vitamin C, E), and evaluate co-factor element profiles zinc (Zn), copper (Cu), iron (Fe) in the serum of Iraqi gastric cancer patients. By elucidating these relationships, the research aims to enhance knowledge on oxidative stress, dietary factors, and potential therapeutic approaches for managing nutritional imbalances in this cohort.
The study included 60 gastric cancer patients and 60 matched controls. Blood samples were collected and serum was extracted for analysis of TOS, TAS, Vitamin C, Vitamin E, copper, zinc, and iron levels. Measurements were conducted using spectrophotometry and ELISA kits from various companies. Statistical analysis was performed with IBM SPSS 25.0, presenting results as means ± SD, and differences between groups were assessed via independent samples t-test at p < 0.05 and p ≤ 0.01.
The study results indicated statistically significant differences between gastric cancer patients and controls in various biomarkers, with all comparisons showing p-values less than 0.001. This suggests strong evidence for the observed differences between the two groups in (TOS), (TAS), vitamins C and E, Cu, Zn, and Fe levels. These relationships underscore the intricate connections among biomarkers in gastric cancer pathophysiology.
The study underscores the intricate relationship between oxidative stress, antioxidant depletion, and micronutrient imbalances in gastric cancer. These findings offer valuable insights into the disease's pathophysiology, indicating potential diagnostic biomarkers. Further research is warranted to unravel underlying mechanisms and explore clinical applications for early gastric cancer diagnosis.
本研究旨在分析氧化应激标志物总氧化剂状态（TOS）和总抗氧化状态（TAS），评估抗氧化剂水平（维生素 C、E），并评估血清中佐剂元素锌（Zn）、铜（Cu）、铁（Fe）的谱。通过阐明这些关系，研究旨在提高对氧化应激、饮食因素以及管理该队列中营养失衡的潜在治疗方法的认识。
本研究纳入了 60 例胃癌患者和 60 例匹配的对照。采集血样并提取血清，用于分析 TOS、TAS、维生素 C、维生素 E、铜、锌和铁水平。使用来自不同公司的分光光度法和 ELISA 试剂盒进行测量。采用 IBM SPSS 25.0 进行统计分析，结果表示为均数±标准差，通过独立样本 t 检验评估组间差异，p<0.05 和 p≤0.01 有统计学意义。
研究结果表明，胃癌患者与对照组之间在多种生物标志物上存在统计学显著差异，所有比较的 p 值均小于 0.001。这表明两组之间（TOS）、（TAS）、维生素 C 和 E、Cu、Zn 和 Fe 水平存在明显差异，这表明两组之间存在明显差异。这些关系突出了胃癌病理生理学中生物标志物之间的复杂联系。
本研究强调了氧化应激、抗氧化剂耗竭和胃癌中微量营养素失衡之间的复杂关系。这些发现为该疾病的病理生理学提供了有价值的见解，并表明了潜在的诊断生物标志物。需要进一步研究以揭示潜在的机制，并探索用于早期胃癌诊断的临床应用。
