Ouyang Fang, Guo Honggang, Xie Wen, Song Yingying, Yan Zhuo, Peng Zhenqing, Zhang Yongsheng, Qu Ritao, Xin Huaigen, Yuan Zheming, Xiao Zhishu, Men Xingyuan
College of Life Sciences, Hebei University, Baoding, Hebei, China.
Key Laboratory of Animal Biodiversity Conservation and Integrated Pest Management, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
J Econ Entomol. 2025 Feb 11;118(1):172-183. doi: 10.1093/jee/toae257.
The solitary bee Osmia excavata (Hymenoptera: Megachilidae) is a key pollinator managed on a large scale. It has been widely used for commercial pollination of fruit trees, vegetables, and other crops with high efficiency in increasing the crop seeding rate, yield, and seed quality in Northern hemisphere. Here, a high-quality chromosome-level genome of O. excavata was generated using PacBio sequencing along with Hi-C technology. The genome size was 207.02 Mb, of which 90.25% of assembled sequences were anchored to 16 chromosomes with a contig N50 of 9,485 kb. Approximately 186.83 Mb, accounting for 27.93% of the genome, was identified as repeat sequences. The genome comprises 12,259 protein-coding genes, 96.24% of which were functionally annotated. Comparative genomics analysis suggested that the common ancestor of O. excavata and Osmia bicornis (Hymenoptera: Megachilidae) lived 8.54 million years ago. Furthermore, cytochrome P450 family might be involved in the responses of O. excavata to low-temperature stress. Taken together, the chromosome-level genome assembly of O. excavata provides in-depth knowledge and will be a helpful resource for the pollination biology research.
独居蜂掘土 Osmia excavata（膜翅目：切叶蜂科）是一种被大规模管理的关键传粉者。它已被广泛用于北半球果树、蔬菜和其他作物的商业授粉，在提高作物播种率、产量和种子质量方面效率很高。在此，利用PacBio测序技术和Hi-C技术构建了高质量的掘土 Osmia excavata 染色体水平基因组。基因组大小为207.02 Mb，其中90.25%的组装序列被锚定到16条染色体上，重叠群N50为9485 kb。约186.83 Mb，占基因组的27.93%，被鉴定为重复序列。该基因组包含12259个蛋白质编码基因，其中96.24%具有功能注释。比较基因组学分析表明，掘土 Osmia excavata 和双角 Osmia bicornis（膜翅目：切叶蜂科）的共同祖先生活在854万年前。此外，细胞色素P450家族可能参与了掘土 Osmia excavata 对低温胁迫的反应。总之，掘土 Osmia excavata 的染色体水平基因组组装提供了深入的知识，将成为传粉生物学研究的有用资源。
