Key Laboratory of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, Beijing, P.R. China.
College of Biological Sciences, University of Chinese Academy of Sciences, Beijing, P.R. China.
Genome Biol Evol. 2022 Feb 4;14(2). doi: 10.1093/gbe/evac014.
Anthidiini, a large bee tribe characterized by light-colored maculations, represents nearly 1,000 pollinator species, but no genomes are yet available for this tribe. Here, we report a chromosome-level genome assembly of Anthidium xuezhongi collected from the Tibetan Plateau. Using PacBio long reads and Hi-C data, we assembled a genome of 189.14 Mb with 99.94% of the assembly located in 16 chromosomes. Our assembly contains 23 scaffolds, with the scaffold N50 length of 12.53 Mb, and BUSCO completeness of 98.70% (n = 1,367). We masked 25.98 Mb (13.74%) of the assembly as repetitive elements, identified 385 noncoding RNAs, and predicted 10,820 protein-coding genes (99.20% BUSCO completeness). Gene family evolution analyses identified 9,251 gene families, of which 31 gene families experienced rapid evolution. Interspecific chromosomal variation among A. xuezhongi, Bombus terrestris, and Apis mellifera showed strong chromosomal syntenic relationships. This high-quality genome assembly is a valuable resource for evolutionary and comparative genomic analyses of bees.
Anthidiini 是一个大型的蜜蜂部落，以浅色的色斑为特征，代表了近 1000 种传粉物种，但该部落目前还没有基因组数据。在这里，我们报告了来自青藏高原的Anthidium xuezhongi 的染色体水平基因组组装。我们使用 PacBio 长读长和 Hi-C 数据组装了一个 189.14 Mb 的基因组，其中 99.94%的组装位于 16 条染色体上。我们的组装包含 23 个 scaffolds，其中 N50 长度为 12.53 Mb，BUSCO 完整性为 98.70%（n=1367）。我们将 25.98 Mb（13.74%）的组装区域屏蔽为重复元件，鉴定了 385 个非编码 RNA，并预测了 10820 个蛋白质编码基因（BUSCO 完整性为 99.20%）。基因家族进化分析鉴定了 9251 个基因家族，其中 31 个基因家族经历了快速进化。A. xuezhongi、Bombus terrestris 和 Apis mellifera 之间的种间染色体变异显示出强烈的染色体共线性关系。这个高质量的基因组组装为蜜蜂的进化和比较基因组学分析提供了有价值的资源。
