Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA.
Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; Division of Gastroenterology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA; Clinical and Translational Epidemiology Unit, Massachusetts General Hospital, Boston, MA 02114, USA.
Cell Host Microbe. 2024 Nov 13;32(11):1887-1896.e8. doi: 10.1016/j.chom.2024.10.002. Epub 2024 Oct 28.
Gut microbial catechol dehydroxylases are a largely uncharacterized family of metalloenzymes that potentially impact human health by metabolizing dietary polyphenols. Here, we use metatranscriptomics (MTX) to identify highly transcribed catechol-dehydroxylase-encoding genes in human gut microbiomes. We discover a prevalent, previously uncharacterized catechol dehydroxylase (Gp Hcdh) from Gordonibacter pamelaeae that dehydroxylates hydrocaffeic acid (HCA), an anti-inflammatory gut microbial metabolite derived from plant-based foods. Further analyses suggest that the activity of Gp Hcdh may reduce anti-inflammatory benefits of polyphenol-rich foods. Together, these results show the utility of combining MTX analysis and biochemical characterization for gut microbial enzyme discovery and reveal a potential link between host inflammation and a specific polyphenol-metabolizing gut microbial enzyme.
肠道微生物儿茶酚脱羟酶是一大类尚未被充分描述的金属酶，它们通过代谢膳食多酚，可能会对人类健康产生影响。在这里，我们使用宏转录组学（MTX）来鉴定人类肠道微生物组中高度转录的儿茶酚脱羟酶编码基因。我们发现了一种来自戈登氏菌的普遍存在的、以前未被描述的儿茶酚脱羟酶（Gp Hcdh），它可以使咖啡鞣酸（HCA）脱羟基，HCA 是一种源自植物性食物的抗炎肠道微生物代谢物。进一步的分析表明，Gp Hcdh 的活性可能会降低富含多酚的食物的抗炎益处。总之，这些结果表明，将 MTX 分析和生化特性分析相结合用于肠道微生物酶的发现具有实用性，并揭示了宿主炎症与特定的多酚代谢肠道微生物酶之间的潜在联系。
