Druzhkova Irina, Bylinskaya Kseniya, Plekhanov Anton, Kostyuk Alexey, Kirillin Mikhail, Perekatova Valeriya, Khilov Aleksandr, Orlova Anna, Polozova Anastasiya, Komarova Anastasiya, Lisitsa Uliyana, Sirotkina Marina, Shirmanova Marina, Turchin Ilya
Institute of Experimental Oncology and Biomedical Technologies, Privolzhsky Research Medical University, Nizhny Novgorod, Russia.
Department for Radiophysical methods in medicine, Institute of Applied Physics of Russian Academy of Sciences, Nizhny Novgorod, Russia.
J Biophotonics. 2024 Oct 29:e202400339. doi: 10.1002/jbio.202400339.
The effects of cytotoxic chemotherapy on tumor vasculature and oxygenation are in the focus of modern investigations because vascular structure and distribution of oxygen influence tumor behavior and treatment response. The aim of our study was to monitor changes in the vascular component of colorectal tumor xenografts induced by a clinical combination of chemotherapy drugs FOLFOX in vivo using two complementary techniques: diffuse reflectance spectroscopy (DRS) and optical coherence tomography-based microangiography (OCT-MA). These techniques revealed a slower decrease in tumor blood oxygenation in treated tumors as compared to untreated ones, faster suppression of tumor vasculature perfusion and increase in water content as a result of treatment, and decrease in total hemoglobin in untreated tumors. Immunohistochemical analysis of hypoxia-inducible factor HIF-2α detected tissue hypoxia as a consequence of inappropriate oxygen supply in the treated tumors. The obtained results show the prospects for monitoring of treatment efficacy using DRS and OCT-MA.
细胞毒性化疗对肿瘤血管系统和氧合作用的影响是现代研究的重点，因为血管结构和氧分布会影响肿瘤行为和治疗反应。我们研究的目的是使用两种互补技术，即漫反射光谱法（DRS）和基于光学相干断层扫描的微血管造影术（OCT-MA），在体内监测临床化疗药物FOLFOX联合用药诱导的结直肠癌异种移植瘤血管成分的变化。这些技术显示，与未治疗的肿瘤相比，治疗后的肿瘤血氧合下降较慢，肿瘤血管灌注受抑制更快，治疗导致含水量增加，未治疗的肿瘤总血红蛋白减少。对缺氧诱导因子HIF-2α的免疫组织化学分析检测到，治疗后的肿瘤因供氧不当而出现组织缺氧。所得结果显示了使用DRS和OCT-MA监测治疗效果的前景。
