Metabolomics Research Center for Functional Materials, Kyungsung University, Busan, 48434, Republic of Korea.
Department of Plant Medicine, College of Agriculture, Life and Environment Science, Chungbuk National University, Cheongju, 28644, Republic of Korea.
Sci Rep. 2024 Oct 29;14(1):25948. doi: 10.1038/s41598-024-77436-0.
Although ethyl formate (EF) fumigant and low temperature applications are widely used for pest management, studies related to their mechanisms of action and subsequent metabolic changes in Drosophila suzukii models are still unclear. In this study, a comparative metabolome analysis was performed to investigate the major metabolites modified by EF and low temperature and how they are related to and affect insect physiology. Most of the identified metabolites function in metabolic pathways related to the biosynthesis of amino acids, nucleotides and cofactors. In addition, a combined treatment with EF and low temperature significantly altered the tricarboxylic acid cycle (TCA) and the levels of the purine and pyrimidine classes of metabolites. Interestingly, the levels of cytochrome P450 and glutathione metabolites involved in detoxification dramatically changed under stress conditions compared to those in the control group.
尽管甲酸乙酯（EF）熏蒸和低温应用广泛用于害虫管理，但与果蝇模型中它们的作用机制和随后的代谢变化相关的研究仍不清楚。在这项研究中，进行了比较代谢组学分析，以研究 EF 和低温修饰的主要代谢物，以及它们如何与昆虫生理学相关并影响昆虫生理学。大多数鉴定出的代谢物在与氨基酸、核苷酸和辅助因子生物合成相关的代谢途径中发挥作用。此外，EF 和低温联合处理显著改变了三羧酸循环（TCA）以及嘌呤和嘧啶类代谢物的水平。有趣的是，与对照组相比，在应激条件下，参与解毒的细胞色素 P450 和谷胱甘肽代谢物的水平发生了显著变化。
