Lee Junbeom, Kim Hyun-Kyung, Jeon Jong-Chan, Seok Seung-Ju, Kim Gil-Hah, Koo Hyun-Na, Lee Dae-Weon
Metabolomics Research Center for Functional Materials, Kyungsung University, Busan 48434, Republic of Korea.
Department of Plant Medicine, College of Agriculture, Life and Environment Science, Chungbuk National University, Cheongju 28644, Republic of Korea.
Metabolites. 2024 Sep 28;14(10):526. doi: 10.3390/metabo14100526.
The mechanisms of action of phosphine are diverse and include neurotoxicity, metabolic inhibition, and oxidative stress; however, its efficacy at low temperatures is unclear. : Comparative metabolomics is suitable for investigating the response of the spotted-wing fly to exposure toward a combination of cold stimuli and fumigant PH. : Under this combined exposure, 52 metabolites exhibiting significant differences in stress were identified and their physiological roles were analyzed in the metabolic pathway. Most metabolites were involved in amino acids, TCA cycle, and nucleic acids. In addition, the alteration levels of cell membrane lipids, such as glycerophospholipids, sphingolipids, and glycerolipids, clearly showed changes in the combined treatment compared to PH and low temperatures alone. Aconitic acid, a component of the TCA cycle, was completely inhibited by the combined treatment. : These results suggest that treatment-specific indicators could be useful biomarkers to indicate the synergistic effects of PH and low temperature on energy metabolism.
磷化氢的作用机制多种多样，包括神经毒性、代谢抑制和氧化应激；然而，其在低温下的效果尚不清楚。比较代谢组学适用于研究斑翅果蝇对冷刺激和熏蒸剂磷化氢联合暴露的反应。在这种联合暴露下，鉴定出52种在应激中表现出显著差异的代谢物，并在代谢途径中分析了它们的生理作用。大多数代谢物参与氨基酸、三羧酸循环和核酸代谢。此外，与单独的磷化氢和低温处理相比，联合处理中细胞膜脂质（如甘油磷脂、鞘脂和甘油脂）的变化水平明显显示出变化。三羧酸循环的组成部分乌头酸在联合处理中被完全抑制。这些结果表明，特定处理指标可能是有用的生物标志物，可指示磷化氢和低温对能量代谢的协同作用。
