Primary Investigation on the Synergistic Effects of Methyl Bromide and 1 °C Cold Treatment for Two-Spotted Spider Mite () and the Citrus Mealybug ().

Since the use of methyl bromide has been prohibited globally because of environmental concerns, several alternative fumigants have been newly developed and applied to fresh products. However, single treatment with a methyl bromide alternative fumigant cannot completely replace methyl bromide treatment for some products because of issues associated with long treatment times and phytotoxicity. In this study, we compared the mortality of two agricultural pests, and , after single treatment with methyl bromide and combined application of methyl bromide and cold treatment to confirm the synergistic effects of chemical and physical treatments. The combined application of methyl bromide and cold treatment was effective against the nymph and adult stages of , but no synergism was observed at the egg stage. For , the required dosage of methyl bromide decreased when methyl bromide treatment was followed by low temperature, possibly because of the susceptibility of to cold treatment. These results indicate that the synergism of fumigants with cold treatment can differ by pest species and growth stage, and further studies on other pests are needed to reduce methyl bromide usage.