Nur Yuspian, Zein Muhammad Ihda Hl, Irkham Irkham, Gaffar Shabarni, Subroto Toto, Hartati Yeni Wahyuni
Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Indonesia.
Laboratory of Research and Development of Farmaka Tropis, Faculty of Pharmacy, Universitas Mulawarman, Indonesia.
ADMET DMPK. 2024 Aug 18;12(4):623-635. doi: 10.5599/admet.2404. eCollection 2024.
Chronic myeloid leukaemia (CML) is one of the most lethal types of leukaemia and can rapidly progress if not treated properly. Therefore, having an effective diagnostic strategy is crucial. Various methods are available for diagnosis, including electrochemical biosensors with aptamer bioreceptors.
In this study, we immobilized the KK1D04 aptamer on a screen-printed carbon electrode (SPCE) supported by CeO nanoparticles (CeONPs) to detect K562 cells, a type of CML cell line. Several parameters were optimized to enhance the aptasensor response using the Box-Behnken experimental design.
The developed aptasensor demonstrated good performance with a limit of detection (LOD) and limit of quantification (LOQ) of 16 cells/mL and 3,882 cells/mL, respectively, in the K562 cell concentration range of 10 to 10 cells/mL. The optimum experimental conditions were an aptamer concentration of 0.8 ppm, an aptamer incubation time of 36 minutes, and a K562 aptamer-cell incubation time of 13 minutes. The aptasensor also exhibits selectivity for K562 cells compared to Vero cells, THP1 cells, and Raji cells.
The aptasensor in this study demonstrated the potential to detect K562 cells. These results could contribute to the advancement of point-of-care (POC) devices for the detection of CML.
慢性髓性白血病（CML）是最致命的白血病类型之一，若治疗不当会迅速进展。因此，拥有有效的诊断策略至关重要。诊断方法多种多样，包括使用适配体生物受体的电化学生物传感器。
在本研究中，我们将KK1D04适配体固定在由二氧化铈纳米颗粒（CeONPs）支撑的丝网印刷碳电极（SPCE）上，以检测K562细胞，这是一种CML细胞系。使用Box-Behnken实验设计优化了几个参数，以增强适配体传感器的响应。
所开发的适配体传感器表现出良好的性能，在10至10⁶个细胞/毫升的K562细胞浓度范围内，检测限（LOD）和定量限（LOQ）分别为16个细胞/毫升和3882个细胞/毫升。最佳实验条件为适配体浓度0.8 ppm、适配体孵育时间36分钟以及K562适配体-细胞孵育时间13分钟。与Vero细胞、THP1细胞和Raji细胞相比，该适配体传感器对K562细胞也具有选择性。
本研究中的适配体传感器显示出检测K562细胞的潜力。这些结果可能有助于推动用于检测CML的即时检测（POC）设备的发展。
