Department of Chemical and Biomolecular Engineering, National University of Singapore, Singapore 117585, Singapore.
Nanoscale. 2024 Nov 28;16(46):21264-21278. doi: 10.1039/d4nr03760j.
The pressing issue of food security amid climate change necessitates innovative agricultural practices, including advanced plant genetic engineering techniques. Efficient delivery of biomolecules such as DNA, RNA, and proteins into plant cells is essential for targeted crop improvements, yet traditional methods face significant barriers. This review discusses the multifaceted challenges of biomolecule delivery into plant cells, emphasizing the limitations of conventional methods. We explore the promise of nanoparticle-mediated delivery systems as a versatile alternative. By highlighting the diverse design parameters used to tune the physical and chemical properties of nanoparticles, we analyze how these factors influence delivery efficacy. Furthermore, we summarize recent advancements in nanoparticle-mediated delivery, showcasing successful examples of DNA, RNA, and protein transport into plant cells. By understanding and optimizing these design parameters, we can enhance the potential of nanoparticle technologies in plant genetic engineering, paving the way for more resilient and productive agriculture.
气候变化背景下的粮食安全紧迫问题需要创新的农业实践，包括先进的植物基因工程技术。高效地将生物分子如 DNA、RNA 和蛋白质递送到植物细胞中对于有针对性的作物改良至关重要，但传统方法面临着重大的障碍。本文讨论了将生物分子递送到植物细胞中所面临的多方面挑战，强调了传统方法的局限性。我们探讨了纳米颗粒介导的递药系统作为一种多功能替代方法的前景。通过突出用于调整纳米颗粒物理和化学性质的各种设计参数，我们分析了这些因素如何影响递药效率。此外，我们总结了纳米颗粒介导的递药的最新进展，展示了将 DNA、RNA 和蛋白质成功递送到植物细胞中的例子。通过理解和优化这些设计参数，我们可以提高纳米颗粒技术在植物基因工程中的潜力，为更具弹性和更高效的农业铺平道路。
