Gray W M
Br J Anaesth. 1986 Mar;58(3):345-52. doi: 10.1093/bja/58.3.345.
A method is described for preparing static standards of per cent concentrations of volatile anaesthetic agents, that is designed to minimize adsorptive losses in the container used for the standards. The extent to which this aim has been realized was investigated for atmospheres of halothane, enflurane and isoflurane, both by monitoring the relative concentration within the container over a period of 30 min after the standard was prepared, and by measuring the absolute concentration of the volatile agents. Gas chromatography was used for both sets of measurements, with liquid standards being used for the absolute measurements. No discernible loss of volatile agent occurred over the 30-min period, and the measured concentrations agreed closely with the predicted values. The standard mixtures prepared by the method were suitable for calibrating a gas chromatograph, and allowed accurate determinations of volatile anaesthetics to be made. The relative standard error of the predicted concentration for a single standard mixture was less than 0.5%, and the calibration accuracy could be further improved by taking the mean chromatograph response to several standard mixtures prepared in succession.
本文描述了一种制备挥发性麻醉剂百分比浓度静态标准品的方法，该方法旨在最大程度减少用于标准品的容器中的吸附损失。对于氟烷、恩氟烷和异氟烷的气体环境，通过在制备标准品后30分钟内监测容器内的相对浓度以及测量挥发性麻醉剂的绝对浓度，研究了这一目标的实现程度。两组测量均使用气相色谱法，绝对测量使用液体标准品。在30分钟内未观察到挥发性麻醉剂有明显损失，测量浓度与预测值非常吻合。通过该方法制备的标准混合物适用于校准气相色谱仪，并能准确测定挥发性麻醉剂。单个标准混合物预测浓度的相对标准误差小于0.5%，通过取气相色谱对连续制备的几种标准混合物的平均响应，校准精度可进一步提高。
