Khanam Arifa, Ijaz Hussain Abdullah, Mohammed Esraa Haji, Nahar Lutfun, Rathore Hassaan A
Department of Chemistry, Government College University Faisalabad, Faisalabad, 38000, Pakistan.
Department of Pharmaceutical Chemistry, College of Pharmacy, University of Hafr Al Batin, Hafr Al Batin, 39524, Saudi Arabia.
Chem Biodivers. 2025 Mar;22(3):e202401728. doi: 10.1002/cbdv.202401728. Epub 2024 Dec 12.
The present study aimed to assess the antioxidant and anti-inflammatory activities of polyphenol-rich extracts of seedless variety of Ziziphus mauritiana (SZM). Reverse Phase High Performance Liquid Chromatography (RP-HPLC) analysis of SZM leaves and fruit extracts in ethanol revealed the presence of sixteen phenolics including chlorogenic acid, p-coumeric acid, gallic acid, kaempferol, rutin and quercetin. Leaf extract showed higher total phenolic and total flavonoid contents (177.6 mg/100 g and 46.2 mg/100 g) than in fruit extract (137.8 mg/100 g and 14.1 mg/100 g). The leaf extract exhibited higher DPPH radical-scavenging activity (63.5 %) than the fruit extract (58.2 %). The anti-inflammatory activity was evaluated on carrageenan-induced rat model and suppression of inflammatory biomarkers (Interleukin-6, Tumor necrosis factor-α and CRP) were studied. The fruit extract exhibited remarkable inhibition (98.1 %) at the dose level of 500 mg/kg body weight (BW), comparable to the standard drug indomethacin (98.4 %). Both extracts suppressed the inflammatory biomarkers and more pronounced results showed by the fruit extract including CRP, IL-6, and TNF-α. The leaf extract demonstrated the higher antioxidant potential as evident from the superoxide dismutase, catalase, malondialdehyde, glutathione peroxidase and glutathione levels. These findings suggest that SZM leaf and fruit extracts possess potential antioxidant and remarkable anti-inflammatory properties and can play a significant role in mitigating oxidative stress.
本研究旨在评估无核毛叶枣（SZM）富含多酚提取物的抗氧化和抗炎活性。对乙醇提取的SZM叶和果实提取物进行反相高效液相色谱（RP-HPLC）分析，结果显示其中含有16种酚类物质，包括绿原酸、对香豆酸、没食子酸、山奈酚、芦丁和槲皮素。叶提取物的总酚含量（177.6 mg/100 g）和总黄酮含量（46.2 mg/100 g）高于果实提取物（137.8 mg/100 g和14.1 mg/100 g）。叶提取物的DPPH自由基清除活性（63.5%）高于果实提取物（58.2%）。在角叉菜胶诱导的大鼠模型上评估抗炎活性，并研究炎症生物标志物（白细胞介素-6、肿瘤坏死因子-α和CRP）的抑制情况。果实提取物在500 mg/kg体重剂量水平下表现出显著的抑制作用（98.1%），与标准药物吲哚美辛（98.4%）相当。两种提取物均能抑制炎症生物标志物，果实提取物的效果更明显，包括对CRP、IL-6和TNF-α的抑制。从超氧化物歧化酶、过氧化氢酶、丙二醛、谷胱甘肽过氧化物酶和谷胱甘肽水平可以明显看出，叶提取物具有更高的抗氧化潜力。这些研究结果表明，SZM叶和果实提取物具有潜在的抗氧化和显著的抗炎特性，在减轻氧化应激方面可发挥重要作用。
