Polaki Sofia, Stamatelopoulou Vasiliki, Kotsou Konstantina, Chatzimitakos Theodoros, Athanasiadis Vassilis, Bozinou Eleni, Lalas Stavros I
Department of Food Science & Nutrition, University of Thessaly, Terma N. Temponera Str., 43100 Karditsa, Greece.
Plants (Basel). 2024 Jul 29;13(15):2105. doi: 10.3390/plants13152105.
L. (HO) is, as one of the most prevalently utilized plants, used in traditional medicine to cure various diseases as well as the in food and cosmetic industries. Moreover, HO is a rich source of polyphenols with potent antioxidant properties. However, the studies on the extraction of such compounds from HO are scanty and sparse. This study aims to optimize the extraction of polyphenols and maximize the antioxidant activity in HO extracts. A comprehensive experimental design was employed, encompassing varied extraction parameters to determine the most effective ones. Alongside conventional stirring (ST), two green approaches, the ultrasonic treatment (US) and the pulsed electric field (PEF), were explored, either alone or in combination. The extracted polyphenolic compounds were identified with a high-performance liquid chromatography-diode array detector (HPLC-DAD). According to the results, the employment of ST along with an ethanolic solvent at 80 °C for 150 min seems beneficial in maximizing the extraction of polyphenols from HO, resulting in extracts with enhanced antioxidant activity. The total polyphenol was noted at 70.65 ± 2.76 mg gallic acid equivalents (GAE)/g dry weight (dw) using the aforementioned techniques, and the antioxidant activity was noted as 582.23 ± 16.88 μmol ascorbic acid equivalents (AAE)/g dw (with FRAP method) and 343.75 ± 15.61 μmol AAE/g dw (with the DPPH method). The as-prepared extracts can be utilized in the food and cosmetics industries to bestow or enhance the antioxidant properties of commercial products.
作为最常用的植物之一，L. (HO) 在传统医学中用于治疗各种疾病，同时也应用于食品和化妆品行业。此外，HO富含具有强大抗氧化特性的多酚。然而，关于从HO中提取此类化合物的研究却很少且很零散。本研究旨在优化多酚的提取，并使HO提取物中的抗氧化活性最大化。采用了全面的实验设计，涵盖各种提取参数以确定最有效的参数。除了传统搅拌（ST）外，还探索了两种绿色方法，即超声处理（US）和脉冲电场（PEF），单独使用或组合使用。用高效液相色谱 - 二极管阵列检测器（HPLC - DAD）鉴定提取的多酚化合物。根据结果，在80°C下使用乙醇溶剂并结合ST搅拌150分钟似乎有利于从HO中最大程度地提取多酚，从而得到具有增强抗氧化活性的提取物。使用上述技术测得总多酚为70.65±2.76毫克没食子酸当量（GAE）/克干重（dw），抗氧化活性用铁离子还原抗氧化能力（FRAP）法测得为582.23±16.88微摩尔抗坏血酸当量（AAE）/克dw，用二苯基苦味酰基自由基（DPPH）法测得为343.75±15.61微摩尔AAE/克dw。所制备的提取物可用于食品和化妆品行业，赋予或增强商业产品的抗氧化性能。
