Peri G, Zanaboni F, Rossini S, Mangioni C, Landoni F, Epis A, Mantovani A
Br J Cancer. 1986 Jan;53(1):47-52. doi: 10.1038/bjc.1986.7.
The effect of human peripheral blood monocytes on the SW626 ovarian carcinoma line was investigated in a colony assay in agar, Percoll-enriched monocytes inhibited colony formation by SW626 carcinoma cells at effector-to-target cell (E:T) ratios as low as 0.3:1. In contrast the same effectors had little cytolytic effect in a 48 h thymidine-release assay at E:T ratios as high as 40:1. Monocyte-depleted nonadherent cells had little inhibitory capacity on SW626 colony formation, whereas unseparated mononuclear cells were intermediate between Percoll-enriched monocytes and lymphoid cells. Sorting of cells positive for the monoclonal antibody marker MO2 confirmed the monocytic nature of cells which inhibited colony formation. Ovarian carcinoma cells freshly isolated from 9 patients were heterogenous in their susceptibility to colony inhibition by mononuclear phagocytes. Cells from 4 patients were not inhibited by effector cells and in one subject promotion of colony formation by mononuclear phagocytes was observed. With 4 cell preparations inhibition of colony formation was found as with the SW626 line. Colony assays may provide a useful methodological approach, particularly when effector cells mediate low levels of killing, of doubtful biological significance, in conventional isotope release assays, or when growth promotion is to be evaluated.
在琼脂集落试验中研究了人外周血单核细胞对SW626卵巢癌细胞系的作用。经Percoll富集的单核细胞在效应细胞与靶细胞（E:T）比例低至0.3:1时就能抑制SW626癌细胞的集落形成。相比之下，在48小时胸苷释放试验中，相同的效应细胞在E:T比例高达40:1时几乎没有细胞溶解作用。去除单核细胞的非贴壁细胞对SW626集落形成几乎没有抑制能力，而未分离的单核细胞的抑制能力介于经Percoll富集的单核细胞和淋巴细胞之间。对单克隆抗体标记MO2呈阳性的细胞进行分选，证实了抑制集落形成的细胞具有单核细胞性质。从9名患者新鲜分离的卵巢癌细胞对单核吞噬细胞集落抑制的敏感性存在异质性。来自4名患者的细胞不受效应细胞抑制，在1名受试者中观察到单核吞噬细胞促进集落形成。对于4份细胞制剂，发现其与SW626细胞系一样具有集落形成抑制作用。集落试验可能提供一种有用的方法学途径，特别是当效应细胞在传统同位素释放试验中介导低水平杀伤（其生物学意义存疑）时，或者当要评估生长促进作用时。
