College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, Shandong, China.
PLoS One. 2024 Oct 30;19(10):e0312821. doi: 10.1371/journal.pone.0312821. eCollection 2024.
This study aimed to comprehensively characterize the gut microbiota in diarrheal mink. We conducted Shotgun metagenomic sequencing on samples from five groups of diarrheal mink and five groups of healthy mink. The microbiota α-diversity and Kyoto Encyclopedia of Genes and Genomes (KEGG) orthology did not show significant differences between the groups. However, significant differences were observed in microbiota β-diversity and the function of carbohydrate-active enzymes (CAZymes) between diarrheal and healthy mink. Specifically, The relative abundance of Firmicutes was lower, whereas that of Bacteroidetes was higher in diarrheal mink. Fusobacteria were enriched as invasive bacteria in the gut of diarrheal mink compared with healthy mink. In addition, Escherichia albertii was identified as a new bacterium in diarrheal mink. Regarding functions, nicotinate and nicotinamide metabolism and glycoside hydrolases 2 (GH2) family were the enhanced KEGG orthology and CAZymes in diarrheal mink. Furthermore, the diversity and number of antibiotic-resistant genes were significantly higher in the diarrheal mink group than in the healthy group. These findings enhance our understanding of the gut microbiota of adult mink and may lead to new approaches to the diagnosis and treatment of mink diarrhea.
本研究旨在全面描述腹泻水貂的肠道微生物群。我们对五组腹泻水貂和五组健康水貂的样本进行了 Shotgun 宏基因组测序。各组之间的微生物多样性α和京都基因与基因组百科全书（KEGG）直系同源没有显著差异。然而，腹泻和健康水貂之间的微生物多样性β和碳水化合物活性酶（CAZymes）的功能存在显著差异。具体而言，腹泻水貂中厚壁菌门的相对丰度较低，拟杆菌门的相对丰度较高。梭杆菌属作为侵袭性细菌在腹泻水貂的肠道中富集，而不是健康水貂。此外，鉴定出大肠杆菌埃伯蒂氏菌是腹泻水貂中的一种新细菌。关于功能，烟酸和烟酰胺代谢和糖苷水解酶 2（GH2）家族是腹泻水貂中增强的 KEGG 直系同源和 CAZymes。此外，腹泻水貂组的抗生素抗性基因的多样性和数量明显高于健康组。这些发现提高了我们对成年水貂肠道微生物群的理解，并可能为水貂腹泻的诊断和治疗提供新方法。
