School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing, 210046, Jiangsu, China.
School of Chemical Engineering, Yangzhou Polytechnic Institute, Yangzhou, 225127, Jiangsu, China.
Curr Microbiol. 2024 Oct 30;81(12):434. doi: 10.1007/s00284-024-03958-5.
The genus Serratia is a typical red bacterium involved in prodigiosin synthesis. Here, we report the genome sequence of Serratia rubidaea XU1, which was isolated from radiation-contaminated soil in Xinjiang, China. The genome of XU1 is composed of 4,972,898 base pairs with a GC content of 59.25%. The genome sequence contains 4707 genes and encodes 4573 proteins, 79 tRNAs, and 17 rRNAs. The prodigiosin biosynthesis gene cluster was identified and analyzed, showing a sequence similarity of 85.55-96.02% with Serratia rubidaea. After optimizing the biosynthesis process, XU1 was able to achieve a maximum titer of 574 units/cell of prodigiosin at a pH of 7.5 and a temperature of 25 °C for 36 h. Glycerol at 20 g/L and beef extract at 5 g/L were used as the carbon and nitrogen sources, respectively. Prodigiosin extracted from XU1 demonstrated inhibition of Escherichia coli, Staphylococcus aureus, Enterococcus faecalis and Pseudomonas aeruginosa. The availability of the sequenced genome of XU1 will be greatly beneficial and contribute to complementary studies on the biosynthetic mechanisms of prodigiosin.
色杆菌属是一种典型的红色细菌，参与灵菌红素的合成。在这里，我们报告了来自中国新疆辐射污染土壤的粘质沙雷氏菌 XU1 的基因组序列。XU1 的基因组由 4972898 个碱基对组成，GC 含量为 59.25%。基因组序列包含 4707 个基因，编码 4573 种蛋白质、79 种 tRNA 和 17 种 rRNA。鉴定并分析了灵菌红素生物合成基因簇，与粘质沙雷氏菌的序列相似性为 85.55-96.02%。优化生物合成过程后，XU1 在 pH7.5、温度 25°C 下培养 36 小时，可达到 574 单位/细胞的灵菌红素最大产量。20g/L 的甘油和 5g/L 的牛肉浸膏分别用作碳源和氮源。从 XU1 中提取的灵菌红素对大肠杆菌、金黄色葡萄球菌、粪肠球菌和铜绿假单胞菌具有抑制作用。XU1 测序基因组的可用性将极大地有益于灵菌红素生物合成机制的互补研究。
