Zheng Yixin, Zhao Jinrong, Nie Xinyu, Chitrakar Bimal, Gao Jie, Sang Yaxin
Hebei Agricultural University, No.2596, Lekai South Street, Baoding, Hebei 86-071000, China.
Hebei Agricultural University, No.2596, Lekai South Street, Baoding, Hebei 86-071000, China.
Int J Biol Macromol. 2024 Dec;282(Pt 4):137031. doi: 10.1016/j.ijbiomac.2024.137031. Epub 2024 Oct 28.
The bacterial ability to adhere and colonize in the gut is a key prerequisite to become a probiotic. Lactobacillus spp. surface layer proteins (SLPs) play an important role for such functions in the human body. Interestingly, all SLPs in spite of their structural variation promote adhesion and colonization. A clear understanding about the binding sites of SLPs with the host and their binding modes would help to precisely reveal the process of Lactobacillus spp.-host interaction. Therefore, in this paper, we have sorted out the Lactobacillus spp. SLPs and their adhesion sites in human intestinal cells. Such SLPs included surface layer protein, motif proteins, binding proteins and moonlighting proteins, while enterocyte adhesion receptors included transmembrane glycoproteins and extracellular matrix proteins. We also summarized the tools to assess the adhesion by Lactobacillus spp. Finally, we recommended that three-dimensional cell models and intestinal microarrays could be major tools for assessing adhesion in the future.
细菌在肠道中黏附和定殖的能力是成为益生菌的关键先决条件。乳酸杆菌属的表层蛋白（SLPs）在人体的此类功能中发挥着重要作用。有趣的是，尽管所有SLPs的结构存在差异，但它们都能促进黏附和定殖。清楚了解SLPs与宿主的结合位点及其结合模式将有助于精确揭示乳酸杆菌属与宿主的相互作用过程。因此，在本文中，我们梳理了乳酸杆菌属的SLPs及其在人肠道细胞中的黏附位点。此类SLPs包括表层蛋白、基序蛋白、结合蛋白和兼性蛋白，而肠上皮细胞黏附受体包括跨膜糖蛋白和细胞外基质蛋白。我们还总结了评估乳酸杆菌属黏附的工具。最后，我们建议三维细胞模型和肠道微阵列可能是未来评估黏附的主要工具。
