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β-内酰胺酶 Bdo_10846 在 中的毒力和诱导苹果植株疣状症状中发挥重要作用。

The Cutinase Bdo_10846 Play an Important Role in the Virulence of and in Inducing the Wart Symptom on Apple Plant.

机构信息

College of Plant Protection and Key Lab of Pest Monitoring and Green Management, MOA, China Agricultural University, Beijing 100193, China.

出版信息

Int J Mol Sci. 2021 Feb 14;22(4):1910. doi: 10.3390/ijms22041910.

DOI:10.3390/ijms22041910
PMID:33673023
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7918748/
Abstract

is a pathogen with worldwide distribution, infecting hundreds of species of economically important woody plants. It infects and causes various symptoms on apple plants, including wart and canker on branches, twigs, and stems. However, the mechanism of warts formation is unclear. In this study, we investigated the mechanism of wart formation by observing the transection ultrastructure of the inoculated cortical tissues at various time points of the infection process and detecting the expression of genes related to the pathogen pathogenicity and plant defense response. Results revealed that wart induced by consisted of proliferous of phelloderm cells, the newly formed secondary phellem, and the suberized phelloderm cells surrounding the invading mycelia. The qRT-PCR analysis revealed the significant upregulation of apple pathogenesis-related and suberification-related genes and a pathogen cutinase gene . The knockout transformants showed reduced cutinase activity and decreased virulence. Transient expression of Bdo_10846 in induced ROS burst, callose formation, the resistance of to , and significant upregulation of the plant pathogenesis-related and suberification-related genes. Additionally, the enzyme activity is essential for the induction. Virus-induced gene silencing demonstrated that the and expression were required for the Bdo_10846 induced defense response in . These results revealed the mechanism of wart formation induced by invasion and the important roles of the cutinase Bdo_10846 in pathogen virulence and in inducing plant immunity.

摘要

是一种具有世界分布的病原体，感染了数百种具有重要经济价值的木本植物。它感染并引起苹果植株的各种症状，包括树枝、小枝和茎上的疣和溃疡。然而，疣形成的机制尚不清楚。在这项研究中，我们通过观察接种后皮层组织在感染过程中不同时间点的横切面超微结构，并检测与病原体致病性和植物防御反应相关的基因表达，研究了疣形成的机制。结果表明，由 引起的疣由增生的栓内层细胞、新形成的次生木栓层和围绕入侵菌丝的木质化栓内层细胞组成。qRT-PCR 分析显示，苹果病程相关和木质化相关基因以及病原体角质酶基因 显著上调。 基因敲除转化体显示角质酶活性降低，毒力降低。在 中瞬时表达 Bdo_10846 诱导 ROS 爆发、胼胝质形成、对 的抗性增强，以及植物病程相关和木质化相关基因的显著上调。此外，该酶活性对于诱导是必需的。病毒诱导的基因沉默表明，在 中， 和 表达是 Bdo_10846 诱导防御反应所必需的。这些结果揭示了 入侵引起疣形成的机制以及角质酶 Bdo_10846 在病原体毒力和诱导植物免疫中的重要作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2738/7918748/b10ae224f101/ijms-22-01910-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2738/7918748/5766e3d0d711/ijms-22-01910-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2738/7918748/ab9b9f3282c0/ijms-22-01910-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2738/7918748/49bda636fa44/ijms-22-01910-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2738/7918748/a02f2d05a34f/ijms-22-01910-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2738/7918748/b41600b96635/ijms-22-01910-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2738/7918748/c2c7e44ed5b3/ijms-22-01910-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2738/7918748/b10ae224f101/ijms-22-01910-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2738/7918748/5766e3d0d711/ijms-22-01910-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2738/7918748/ab9b9f3282c0/ijms-22-01910-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2738/7918748/49bda636fa44/ijms-22-01910-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2738/7918748/a02f2d05a34f/ijms-22-01910-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2738/7918748/b41600b96635/ijms-22-01910-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2738/7918748/c2c7e44ed5b3/ijms-22-01910-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2738/7918748/b10ae224f101/ijms-22-01910-g007.jpg

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