Ma Xiaocao, Zhao Bichun, Wang Chao, Sun Manqiang, Dai Yawen, E Lingling, Gao Mingzhu, Liu Xiangwei, Jia Yali, Yue Wen, Liu Hongchen
School of Medicine, Nankai University, Tianjin 300071, China.
Institute of Stomatology and Oral Maxilla Facial Key Laboratory, Chinese PLA General Hospital, Beijing 100853, China.
Stem Cells Int. 2024 Oct 23;2024:7045341. doi: 10.1155/2024/7045341. eCollection 2024.
Dental trauma is highly prevalent in children and adolescents, alongside tooth decay. This condition mainly induces pulp contamination, pulp necrosis, and tooth avulsion in the clinical context. The disturbance to root growth is prone to occur in immature permanent teeth. However, conventional endodontic treatment may not achieve favorable outcomes in these cases, necessitating conducting relevant exploration. Therefore, this study was performed to examine the impact of Annexin A1 (ANXA1) on the vascular repair of dental pulp using human dental pulp stem cells (DPSCs). Specifically, RNA sequencing (RNA-Seq) and functional clustering analyses were employed to identify key genes involved in pulp regeneration. ANXA1 was detected in DPSCs and may correlate with pulp restoration. However, it remains undefined about the potential of ANXA1 to promote the angiogenetic differentiation of DPSCs. The results of this study revealed that the addition of ANXA1 significantly enhanced the secretion of vascular endothelial growth factor-A (VEGF-A) in DPSCs. Moreover, the incubation of DPSCs with ANXA1 resulted in a higher expression level of endothelial markers and promoted vessel formation through the upregulation of the phosphorylated p38 (p-p38) pathway. The in vivo results corroborated that the ANXA1 group exhibited more blood vessels and an increased ratio of positive staining for CD31. In conclusion, these findings indicate that ANXA1 enhances the in vivo and in vitro vascularization of DPSCs, and the activation of p-p38 may play a pivotal role in mediating the differentiation process.
与龋齿一样，牙外伤在儿童和青少年中非常普遍。在临床情况下，这种情况主要会导致牙髓污染、牙髓坏死和牙齿脱位。未成熟恒牙容易出现牙根生长紊乱。然而，传统的牙髓治疗在这些病例中可能无法取得良好的效果，因此有必要进行相关探索。因此，本研究旨在使用人牙髓干细胞（DPSCs）研究膜联蛋白A1（ANXA1）对牙髓血管修复的影响。具体而言，采用RNA测序（RNA-Seq）和功能聚类分析来鉴定参与牙髓再生的关键基因。在DPSCs中检测到ANXA1，它可能与牙髓修复相关。然而，ANXA1促进DPSCs血管生成分化的潜力仍不明确。本研究结果表明，添加ANXA1可显著增强DPSCs中血管内皮生长因子-A（VEGF-A）的分泌。此外，用ANXA1培养DPSCs导致内皮标志物表达水平更高，并通过上调磷酸化p38（p-p38）途径促进血管形成。体内结果证实，ANXA1组显示出更多的血管，CD31阳性染色比例增加。总之，这些发现表明ANXA1增强了DPSCs在体内和体外的血管化，p-p38的激活可能在介导分化过程中起关键作用。
