Institute for Global Food Security, School of Biological Sciences, Queens University Belfast , Belfast BT9 5BN, United Kingdom.
Centre for Synthesis and Chemical Biology, School of Chemistry, University College Dublin , Dublin D04 V1W8, Ireland.
J Agric Food Chem. 2017 Aug 23;65(33):7186-7191. doi: 10.1021/acs.jafc.7b02029. Epub 2017 Aug 14.
Ongoing consumer concerns over using synthetic additives in foods has strongly influenced efforts worldwide to source suitable natural alternatives. In this study, the antibacterial efficacy of polyacetylene and glucosinolate compounds was evaluated against both Gram positive and Gram negative bacterial strains. Falcarinol [minimum inhibitory concentration (MIC) = 18.8-37.6 μg/mL] demonstrated the best overall antibacterial activity, while sinigrin (MIC = 46.9-62.5 μg/mL) was the most active glucosinolate compound. High-performance liquid chromatography with a diode array detector analysis showed falcarinol [85.13-244.85 μg/g of dry weight (DW)] to be the most abundant polyacetylene within six of the eight carrot (Daucus carota) cultivars investigated. Meanwhile, sinigrin (100.2-244.3 μg/g of DW) was the most abundant glucosinolate present within the majority of broccoli (Brassica oleracea) cultivars investigated using ultra performance liquid chromatography-tandem mass spectrometry analysis. The high abundance of both falcarinol and sinigrin within these respective species suggests that they could serve as potential sources of natural antibacterial agents for use as such in food products.
消费者对食品中使用合成添加剂的持续关注强烈影响了全球范围内寻找合适天然替代品的努力。在这项研究中，评估了聚乙炔和硫代葡萄糖苷化合物对革兰氏阳性和革兰氏阴性菌的抗菌功效。法卡林醇[最小抑菌浓度 (MIC) = 18.8-37.6μg/mL]表现出最佳的整体抗菌活性，而黑芥子硫苷酸钾 (MIC = 46.9-62.5μg/mL)是最活跃的硫代葡萄糖苷化合物。高效液相色谱-二极管阵列检测器分析表明，在研究的八个胡萝卜 (Daucus carota) 品种中，有六个品种的法卡林醇[85.13-244.85μg/g 干重 (DW)]含量最高。同时，超高效液相色谱-串联质谱分析表明，在研究的大多数花椰菜 (Brassica oleracea) 品种中，黑芥子硫苷酸钾 (100.2-244.3μg/g DW)是含量最丰富的硫代葡萄糖苷。这两种物质在各自物种中的高含量表明，它们可能成为食品中天然抗菌剂的潜在来源。
