Tropical Feed Resources Research and Development Center (TROFREC), Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen, Thailand.
Department of Animal Science, Faculty of Agriculture and Natural Resources, Rajamangala University of Technology, Tawan-Ok, Chonburi, Thailand.
PLoS One. 2024 Oct 31;19(10):e0312575. doi: 10.1371/journal.pone.0312575. eCollection 2024.
The objective was to assess the supplementation with microencapsulation of hemp leaf extract (mHLE) utilized as a rumen enhancer on in vitro rumen fermentation and to enhance the bioavailability of active compounds for antimicrobial action, particularly in protozoa and methanogen populations. The feed treatments were totally randomized in the experimental design, with different levels of mHLE diet supplemented at 0, 4, 6 and 8% of total DM substrate and added to an R:C ratio of 60:40. During fermentation, gas kinetics production, nutrient degradability, ammonia nitrogen concentration, volatile fatty acid (VFA) profiles, methane production, and the microbial population were measured. The supplemented treatment at 6% of total DM substrate affected reductions in gas kinetics, cumulative gas production, and volatile fatty acid profiles, especially the acetate and acetate to propionate ratio. Whereas propionate proportion and total volatile fatty acid concentration were enhanced depending on the increase of nutrients in vitro dry matter degradability (IVDMD) after 12 h of post-fermentation at a R:C ratio of 60:40 (P < 0.05). Consequently, mHLE addition resulted in optimal ruminal pH and increased nutrient degradability, followed by ammonia nitrogen concentrations (P < 0.05), which were enhanced by dominant cellulolytic bacteria, particularly Ruminococcus albus and Ruminococcus flavefaciens, which showed the highest growth rates in the rumen ecology. Therefore, mHLE, a rich phytonutrient feed additive, affected the methanogen population, reduced the calculated methane production and can be a potential supplement in the ruminant diet.
本研究旨在评估作为瘤胃增强剂利用的微囊化大麻叶提取物（mHLE）补充物对体外瘤胃发酵的影响，并提高活性化合物的生物利用度以发挥抗菌作用，特别是针对原虫和产甲烷菌群体。在实验设计中，采用完全随机化处理饲料，在日粮中添加不同水平的 mHLE（0、4、6 和 8%总干物质），并添加到 R:C 比为 60:40。在发酵过程中，测量了气体动力学产量、养分降解率、氨氮浓度、挥发性脂肪酸（VFA）谱、甲烷产量和微生物种群。在总干物质底物的 6%添加水平下，影响了气体动力学、累积气体产量和挥发性脂肪酸谱的降低，特别是乙酸和乙酸与丙酸的比例。而丙酸比例和总挥发性脂肪酸浓度随着体外干物质降解率（IVDMD）的增加而增加，在 R:C 比为 60:40 后 12 小时的发酵后（P<0.05）。因此，mHLE 的添加导致瘤胃 pH 值最佳化和养分降解率提高，随后氨氮浓度也提高（P<0.05），这是由优势纤维素分解菌（特别是 Ruminococcus albus 和 Ruminococcus flavefaciens）引起的，它们在瘤胃生态系统中表现出最高的生长速度。因此，mHLE 作为一种富含植物营养素的饲料添加剂，影响了产甲烷菌群体，降低了计算出的甲烷产量，可能是反刍动物日粮的潜在补充物。
