Potokiri Augustina, Omeiza Noah A, Ajayi Abayomi M, Adeleke Paul A, Alagbonsi Abdullateef I, Iwalewa Ezekiel O
Department of Pharmacology and Therapeutics, Neuropharmacology and Toxicology Unit, College of Medicine, University of Ibadan, Ibadan, Nigeria.
Taiwan International Graduate Program in Interdisciplinary Neuroscience, Academia Sinica, Taipei, Taiwan.
Curr Res Physiol. 2024 Oct 15;7:100132. doi: 10.1016/j.crphys.2024.100132. eCollection 2024.
The therapeutic potential of yeast in the management of depression is unknown. Thus, we evaluated the modulatory effect of nutritional yeast supplementation on antidepressant activity of fluoxetine in mice models of depressive-like behaviors (DLB).
A total of 112 mice were divided into 16 groups (n = 7 each) for a 3-stage study. Stage I (non-DLB study) had groups Ia (10 mL/kg vehicle), Ib (20 mg/kg fluoxetine), Ic - If (2% yeast diet for all, but Id - If additionally received 5 mg/kg, 10 mg/kg, and 20 mg/kg fluoxetine respectively). Stage II (lipopolysaccharide [LPS] model of DLB) had groups IIa - IIb (10 mL/kg vehicle), IIc (20 mg/kg fluoxetine), IId (yeast) and IIe (yeast + 20 mg/kg fluoxetine). After these treatments for 24 days, animals in IIb - IIe received 0.83 mg/kg of LPS on the 25th day. Except for group IIIa (10 mL/kg vehicle), animals in other groups of stage III (unpredictable chronic mild stress [UCMS] model) were exposed to UCMS for 24 days along with 10 mL/kg vehicle (IIIb), 20 mg/kg fluoxetine (IIIc), yeast (IIId), or yeast + fluoxetine (IIIe).
Yeast and fluoxetine attenuated LPS- and UCMS-induced immobility, derangement of oxido-inflammatory (TNF-α, IL-6, NO, MDA, SOD, GSH, CAT, and AChE) and CREB/MAPK pathways. While fluoxetine had more potent effect than yeast when used separately, pre-treatment of mice with their combination had more pronounced effect than either of them.
Yeast supplementation improves the antidepressant activity of fluoxetine in mice by modulating oxido-inflammatory, CREB, and MAPK pathways.
酵母在抑郁症治疗中的潜力尚不清楚。因此，我们评估了补充营养酵母对氟西汀在抑郁样行为（DLB）小鼠模型中抗抑郁活性的调节作用。
总共112只小鼠被分为16组（每组n = 7）进行三阶段研究。第一阶段（非DLB研究）有Ia组（10 mL/kg赋形剂）、Ib组（20 mg/kg氟西汀）、Ic - If组（均给予2%酵母饮食，但Id - If组分别额外给予5 mg/kg、10 mg/kg和20 mg/kg氟西汀）。第二阶段（DLB的脂多糖[LPS]模型）有IIa - IIb组（10 mL/kg赋形剂）、IIc组（20 mg/kg氟西汀）、IId组（酵母）和IIe组（酵母 + 20 mg/kg氟西汀）。在这些处理24天后，IIb - IIe组的动物在第25天接受0.83 mg/kg的LPS。除IIIa组（10 mL/kg赋形剂）外，第三阶段（不可预测的慢性轻度应激[UCMS]模型）其他组的动物与10 mL/kg赋形剂（IIIb）、20 mg/kg氟西汀（IIIc）、酵母（IIId）或酵母 + 氟西汀（IIIe）一起接受24天的UCMS。
酵母和氟西汀减轻了LPS和UCMS诱导的不动、氧化炎症（TNF-α、IL-6、NO、MDA、SOD、GSH、CAT和AChE）紊乱以及CREB/MAPK通路紊乱。虽然氟西汀单独使用时比酵母具有更强的作用，但小鼠预先联合使用它们比单独使用任何一种都有更明显的效果。
补充酵母通过调节氧化炎症、CREB和MAPK通路来提高氟西汀在小鼠中的抗抑郁活性。
