Widmaier Mark, Kaiser Antonia, Pandurevic Pontus, Lim Song-I, Döring Andre, Huang Zhiwei, Wenz Daniel, Xiao Ying, Jiang Yun, Xin Lijing
CIBM Center for Biomedical Imaging, Switzerland.
Animal Imaging and Technology, Ecole Polytechnique Federale de Lausanne (EPFL) Lausanne,Switzerland.
Res Sq. 2024 Nov 15:rs.3.rs-5271263. doi: 10.21203/rs.3.rs-5271263/v1.
The creatine kinase (CK) is a key enzyme involved in brain bioenergetics, playing a key role in brain function and the pathogenesis of neurological and psychiatric diseases, but imaging its activity noninvasively in the human brain in vivo remains a significant challenge. This study aims to advance the magnetization transfer (MT)- P magnetic resonance fingerprinting (MRF) for 3D Creatine Kinase Imaging (CKI). The method was implemented and validated on a clinical 7 Tesla MRI scanner. It enables whole-brain mapping of CK reaction rates for the first time, showing robust reproducibility for 25-minute scan sessions. CKI acquisition also provided simultaneous mapping of adenosine triphosphate and phosphocreatine concentration ratios, phosphocreatine longitudinal relaxation time, and maps. Furthermore, a functional CKI (fCKI) study demonstrated the first CK activation map in response to visual stimulation, revealing a mean 15% increase in CK rates in the visual cortex. The novel imaging modalities, CKI and fCKI, have the potential to offer new insights into brain bioenergetics both at rest and during activity.
肌酸激酶（CK）是参与脑生物能量代谢的关键酶，在脑功能以及神经和精神疾病的发病机制中起关键作用，但在人体大脑中进行无创活体成像其活性仍然是一项重大挑战。本研究旨在推进用于三维肌酸激酶成像（CKI）的磁化传递（MT）-P磁共振指纹图谱（MRF）技术。该方法在临床7特斯拉MRI扫描仪上得以实施并验证。它首次实现了全脑CK反应速率成像，在25分钟扫描时段内显示出强大的可重复性。CKI采集还能同时绘制三磷酸腺苷和磷酸肌酸浓度比、磷酸肌酸纵向弛豫时间以及图谱。此外，一项功能性CKI（fCKI）研究展示了首个响应视觉刺激的CK激活图谱，揭示视觉皮层中CK速率平均增加了15%。新型成像模式CKI和fCKI有潜力为静息和活动状态下的脑生物能量代谢提供新的见解。
