Nam Nguyen Nhat, Trinh Thi Ngoc Diep, Do Hoang Dang Khoa, Phan Thang Bach, Trinh Kieu The Loan, Lee Nae Yoon
Biotechnology Center, School of Agriculture and Aquaculture, Tra Vinh University, Tra Vinh City 87000, Vietnam.
Department of Materials Science, School of Applied Chemistry, Tra Vinh University, Tra Vinh City 87000, Vietnam.
Spectrochim Acta A Mol Biomol Spectrosc. 2025 Feb 15;327:125347. doi: 10.1016/j.saa.2024.125347. Epub 2024 Oct 26.
Luminescence nanomaterials (LNMs) have recently received great attention in biological analysis and sensing owing to their key advances in easy design and functionalization with high photostability, luminescence stability, low autofluorescence, and multiphoton capacity. The number of publications surrounding LNMs for biological applications has grown rapidly. LNMs based on Stokes and anti-Stokes shifts are powerful tools for biological analysis. Especially, unique properties of anti-Stokes luminescence such as upconversion nanoparticles (UCNPs) with an implementation strategy to use longer-wavelength excitation sources such as near-infrared (NIR) light can depth penetrate to biological tissue for bioanalysis and bioimaging. We observed that the LNMs-based metal-organic frameworks (MOFs) have been developed and paid attention to the field of bioimaging and luminescence-based sensors, because of their structural flexibility, and multifunctionality for the encapsulation of luminophores. This article provides an overview of innovative LNMs such as quantum dots (QDs), UCNPs, and LMOFs. A brief summary of recent progress in design strategies and applications of LNMs including pH and temperature sensing in biologically responsive platforms, pathogen detection, molecular diagnosis, bioimaging, photodynamic, and radiation therapy published within the past three years is highlighted. It was found that the integrated nanosystem of lab-on-a-chip (LOC) with nanomaterials was rapidly widespread and erupting in interest after the COVID-19 pandemic. The simple operation and close processes of the integration nanosystem together with the optimized size and low energy and materials consumption of biochips and devices allow their trend study and application to develop portable and intelligent diagnostics tools. The last part of this work is the introduction of the utilization use of LNMs in LOC applications in terms of microfluidics and biodevices.
发光纳米材料（LNMs）近年来在生物分析和传感领域备受关注，这得益于其在易于设计和功能化方面取得的关键进展，具有高光稳定性、发光稳定性、低自发荧光和多光子能力。围绕用于生物应用的LNMs的出版物数量迅速增长。基于斯托克斯和反斯托克斯位移的LNMs是生物分析的有力工具。特别是，反斯托克斯发光的独特性质，如具有上转换纳米粒子（UCNPs），其采用使用近红外（NIR）光等长波长激发源的实施策略，可以深度穿透生物组织用于生物分析和生物成像。我们观察到，基于LNMs的金属有机框架（MOFs）因其结构灵活性以及用于封装发光体的多功能性，已在生物成像和基于发光的传感器领域得到开发并受到关注。本文概述了量子点（QDs）、UCNPs和LMOFs等创新型LNMs。重点总结了LNMs在设计策略和应用方面的最新进展，包括过去三年内在生物响应平台中的pH和温度传感、病原体检测、分子诊断、生物成像、光动力和放射治疗等方面的进展。研究发现，纳米材料与芯片实验室（LOC）的集成纳米系统在新冠疫情后迅速普及并引发了人们的兴趣。集成纳米系统的操作简单且流程紧密，再加上生物芯片和设备的尺寸优化、低能耗和低材料消耗，使得它们在开发便携式和智能诊断工具方面具有研究和应用趋势。这项工作的最后一部分介绍了LNMs在微流体和生物设备方面用于LOC应用的情况。
