通过对人类动脉、毛细血管和神经元激活的同时评估来量化神经血管耦合：一项多模态 EEG-fNIRS-TCD 研究。
Quantifying neurovascular coupling through a concurrent assessment of arterial, capillary, and neuronal activation in humans: A multimodal EEG-fNIRS-TCD investigation.
机构信息
Cerebrovascular Concussion Lab, Faculty of Kinesiology, University of Calgary, Alberta, Canada; Human Performance Laboratory, Faculty of Kinesiology, University of Calgary, Calgary, Alberta, Canada; Libin Cardiovascular Institute of Alberta, University of Calgary, Alberta, Canada; Sport Injury Prevention Research Centre, Faculty of Kinesiology, University of Calgary, Calgary, Alberta, Canada; Alberta Children's Hospital Research Institute, University of Calgary, Calgary, Alberta, Canada; Hotchkiss Brain Institute, University of Calgary, Calgary, Alberta, Canada; Integrated Concussion Research Program, University of Calgary, Calgary, Alberta, Canada.
Alberta Children's Hospital Research Institute, University of Calgary, Calgary, Alberta, Canada; Hotchkiss Brain Institute, University of Calgary, Calgary, Alberta, Canada; Integrated Concussion Research Program, University of Calgary, Calgary, Alberta, Canada; Department of Clinical Neurosciences, Cumming School of Medicine, University of Calgary, Calgary, Alberta, Canada.
出版信息
Neuroimage. 2024 Nov 15;302:120910. doi: 10.1016/j.neuroimage.2024.120910. Epub 2024 Oct 30.
BACKGROUND
This study explored a novel multimodal neuroimaging approach to assess neurovascular coupling (NVC) in humans using electroencephalography (EEG), functional near-infrared spectroscopy (fNIRS), and transcranial Doppler ultrasound (TCD).
METHODS
Fifteen participants (nine females; age 19-32) completed concurrent EEG-fNIRS-TCD imaging during motor (finger tapping) and visual ("Where's Waldo?") tasks, with synchronized monitoring of blood pressure, capnography, and heart rate. fNIRS assessed microvascular oxygenation within the frontal, motor, parietal, and occipital cortices, while the middle and posterior cerebral arteries (MCA/PCA) were insonated using TCD. A 16-channel EEG set-up was placed according to the 10-20 system. Wilcoxon signed-rank tests were used to compare physiological responses between the active and resting phases of the tasks, while cross-correlations with zero legs compared cerebral and systemic hemodynamic responses across both tasks.
RESULTS
Time-frequency analysis demonstrated a reduction in alpha and low beta band power in electrodes C3/C4 during finger tapping (p<0.045) and all electrodes during the Waldo task (all p<0.001). During Waldo, cross-correlation analysis demonstrated the change in oxygenated hemoglobin and cerebral blood velocity had a moderate-to-strong negative correlation with systemic physiological influences, highlighting the measured change resulted from neuronal input. Deoxygenated hemoglobin displayed the greatest negative cross-correlation with the MCA/PCA within the motor cortices and visual during the motor and visual tasks, respectively (range:0.54, -0.82).
CONCLUSIONS
This investigation demonstrated the feasibility of the proposed EEG-fNIRS-TCD response to comprehensively assess the NVC response within human, specifically quantifying the real-time temporal synchrony between neuronal activation (EEG), microvascular oxygenation changes (fNIRS), and conduit artery velocity alterations (TCD).
背景
本研究探索了一种新的多模态神经影像学方法，通过脑电图（EEG）、功能近红外光谱（fNIRS）和经颅多普勒超声（TCD）评估人类的神经血管耦合（NVC）。
方法
15 名参与者（9 名女性；年龄 19-32 岁）在进行运动（手指敲击）和视觉（“Where's Waldo？”）任务时完成了同时进行的 EEG-fNIRS-TCD 成像，同时同步监测血压、二氧化碳图和心率。fNIRS 评估了额、运动、顶和枕皮质内的微血管氧合，同时使用 TCD 对大脑中动脉/大脑后动脉（MCA/PCA）进行了探测。根据 10-20 系统放置了 16 通道 EEG 装置。使用 Wilcoxon 符号秩检验比较了任务主动和休息阶段的生理反应，而零滞后交叉相关比较了两个任务中的大脑和全身血液动力学反应。
结果
时频分析表明，在手指敲击时（p<0.045），电极 C3/C4 中的α和低β频段功率降低，而在所有电极上，在 Waldo 任务期间，所有电极的功率均降低（均 p<0.001）。在 Waldo 期间，交叉相关分析表明，氧合血红蛋白和脑血流速度的变化与全身生理影响呈中度至强负相关，这表明所测量的变化是由神经元输入引起的。去氧血红蛋白与运动和视觉任务中运动皮质和视觉的 MCA/PCA 之间具有最大的负交叉相关（范围：0.54，-0.82）。
结论
本研究证明了拟议的 EEG-fNIRS-TCD 反应在全面评估人类 NVC 反应中的可行性，特别是定量测量神经元激活（EEG）、微血管氧合变化（fNIRS）和导管动脉速度变化（TCD）之间的实时时间同步。
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