Torati Sri Ramulu, Slaughter Gymama
Center for Bioelectronics, Old Dominion University, Norfolk, VA 23508, United States.
Center for Bioelectronics, Old Dominion University, Norfolk, VA 23508, United States; Department of Electrical and Computer Engineering, Old Dominion University, Norfolk, VA 23508, United States.
Bioelectrochemistry. 2025 Feb;161:108842. doi: 10.1016/j.bioelechem.2024.108842. Epub 2024 Oct 28.
C-reactive protein (CRP) is a critical biomarker for detecting inflammation and forecasting cardiovascular disease. We present an advanced electrochemical immunosensor utilizing laser-induced graphene (LIG)/MXene-gold nanoparticles (Mx-AuNPs) electrode for CRP detection. The Mx-AuNPs nanocomposite, synthesized via in-situ reduction of HAuCl by MXene, leverages MXene's reducing properties for effective nanoparticle deposition, confirmed through scanning electron microscopy. This electrode demonstrates superior electrochemical performance due to enhanced surface area and synergy between LIG and Mx-AuNPs, improving overall electrode conductivity. The Anti-CRP antibody, immobilized via a cysteamine linker, enables CRP detection. The immunosensor achieves excellent detection across 10 pg mL to 10 µg mL CRP, with a low detection limit of 1.45 pg mL, and shows high selectivity for CRP. This LIG/Mx-AuNPs-based immunosensor is promising for sensitive CRP detection, aiding early cardiovascular disease diagnosis and improving patient outcomes.
C反应蛋白（CRP）是一种用于检测炎症和预测心血管疾病的关键生物标志物。我们展示了一种先进的电化学免疫传感器，它利用激光诱导石墨烯（LIG）/MXene-金纳米颗粒（Mx-AuNPs）电极来检测CRP。通过MXene原位还原HAuCl合成的Mx-AuNPs纳米复合材料，利用MXene的还原特性实现了有效的纳米颗粒沉积，这通过扫描电子显微镜得到了证实。由于表面积增加以及LIG和Mx-AuNPs之间的协同作用，该电极表现出卓越的电化学性能，提高了整体电极的导电性。通过半胱胺连接剂固定的抗CRP抗体实现了CRP的检测。该免疫传感器在10 pg/mL至10 μg/mL的CRP范围内实现了出色的检测，检测限低至1.45 pg/mL，并且对CRP表现出高选择性。这种基于LIG/Mx-AuNPs的免疫传感器在灵敏检测CRP方面具有前景，有助于早期心血管疾病诊断并改善患者预后。
