Sommerfeld Vera, Bennewitz Jörn, Camarinha-Silva Amélia, Feger Martina, Föller Michael, Huber Korinna, Oster Michael, Ponsuksili Siriluck, Schmucker Sonja, Seifert Jana, Stefanski Volker, Wimmers Klaus, Rodehutscord Markus
Institute of Animal Science, University of Hohenheim, Stuttgart, 70599, Germany.
Department of Physiology, University of Hohenheim, Stuttgart, 70599, Germany.
Poult Sci. 2024 Dec;103(12):104407. doi: 10.1016/j.psj.2024.104407. Epub 2024 Oct 10.
The objective of this study was to characterize intestinal phytate degradation and mineral utilization by 2 laying hen strains before and after the onset of egg laying using diets without or with a mineral phosphorus (P) supplement. One offspring of 10 roosters per strain (Lohmann Brown-classic [LB] and Lohmann LSL-classic [LSL]) was sacrificed before (wk 19) and after (wk 24) the onset of egg-laying activity and following 4 wk placement in a metabolic unit. Diets were corn-soybean meal-based and without supplemented P (P-) or with 1 g/kg supplemented P (P+) from monocalcium phosphate. In wk 19 and 24, the blood plasma and digesta of duodenum+jejunum and distal ileum were collected. The concentration of P in blood plasma was higher in hens fed P+ than P- (P < 0.001). In duodenum + jejunum and ileum content, the concentrations of InsP, Ins(1,2,4,5,6)P and Ins(1,2,3,4,5)P were lower in P- than in P+ (P ≤ 0.009). In duodenum+jejunum, the concentrations of InsP, Ins(1,2,4,5,6)P and Ins(1,2,3,4,5)P were lower in wk 24 than 19 and lower in LSL than LB hens (P < 0.001). The concentration of myo-inositol (MI) in duodenum + jejunum content was lower in wk 19 than 24 (P < 0.001). Following a 4-d total excreta collection, the retained amount of P was higher in P+ than P- (P < 0.001). Phosphorus retention was lower in LB hens fed P- than in other treatments (P × strain: P = 0.039). In the jejunal tissue, some genes related to intracellular InsP metabolism were higher expressed in LB than LSL hens. The renunciation of mineral P increased endogenous phytate degradation, but more P was retained with supplemented P. Differences in endogenous phytate degradation between the periods before and after the onset of egg laying might be attributed to different Ca concentrations in intestinal digesta caused by different Ca needs in both periods.
本研究的目的是利用不添加或添加无机磷（P）的日粮，对2个蛋鸡品系产蛋前后肠道植酸盐降解和矿物质利用情况进行表征。每个品系（罗曼褐经典系[LB]和罗曼LSL经典系[LSL]）10只公鸡的后代在产蛋活动开始前（第19周）和开始后（第24周）以及在代谢笼中饲养4周后进行屠宰。日粮以玉米-豆粕为基础，不添加P（P-）或添加1 g/kg来自磷酸二氢钙的P（P+）。在第19周和第24周，采集血浆以及十二指肠+空肠和回肠末端的食糜。采食P+日粮的母鸡血浆中P浓度高于采食P-日粮的母鸡（P < 0.001）。在十二指肠+空肠和回肠内容物中，P-组中肌醇六磷酸（InsP）、肌醇-1,2,4,5,6-五磷酸（Ins(1,2,4,5,6)P）和肌醇-1,2,3,4,5-五磷酸（Ins(1,2,3,4,5)P）的浓度低于P+组（P≤0.009）。在十二指肠+空肠中，InsP、Ins(1,2,4,5,6)P和Ins(1,2,3,4,5)P的浓度在第24周低于第19周，且LSL母鸡低于LB母鸡（P < 0.001）。十二指肠+空肠内容物中肌醇（MI）的浓度在第19周低于第24周（P < 0.001）。在进行4天的总排泄物收集后，P+组的P保留量高于P-组（P < 0.001）。采食P-日粮的LB母鸡的P保留率低于其他处理组（P×品系：P = 0.039）。在空肠组织中，一些与细胞内InsP代谢相关的基因在LB母鸡中的表达高于LSL母鸡。不添加无机P会增加内源性植酸盐降解，但添加P时会保留更多的P。产蛋前后内源性植酸盐降解的差异可能归因于两个时期不同的钙需求导致肠道食糜中钙浓度不同。
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