Institute of Animal Science, University of Hohenheim, 70599, Stuttgart, Germany.
Sci Rep. 2021 Feb 25;11(1):4641. doi: 10.1038/s41598-021-84169-x.
Adaptation to metabolic challenges is an individual process in animals and human, most likely based on genetic background. To identify novel pathways of importance for individual adaptation to a metabolic challenge such as egg production in laying hens, myo-inositol (MI) metabolism and plasma metabolite profiles during the productive lifespan were examined in two genetically different strains, Lohmann Brown-Classic (LB) and LSL-Classic (LSL) hens. They were housed during the productive lifespan and sampled at 10, 16, 24, 30 and 60 weeks of age. The targeted AbsoluteIDQ p180 Kit was used for metabolite profiling in plasma whereas a MI enzymatic kit and ELISAs were used to quantify tissue MI concentrations and MI key enzymes (IMPase 1 and MIOX), respectively. As major finding, kidney MIOX was differently expressed in LB and LSL hens with higher amounts in LB. The onset of egg laying between week 16 and 24 of life span was associated with a clear change in the metabolite profiles, however LSL hens and LB hens adapt differently. Pearson's correlation analyses over all hens at all time points indicated that higher expression of MI degrading enzyme MIOX was related to markers indicating metabolic stress.
动物和人类对代谢挑战的适应是一个个体过程，很可能基于遗传背景。为了确定对代谢挑战（如产蛋）的个体适应的新途径，例如在两个遗传不同的品系中，罗曼褐鸡（LB）和 LSL 鸡（LSL）中检查了肌醇（MI）代谢和产蛋寿命期间的血浆代谢物谱。在产蛋寿命期间对它们进行了饲养，并在 10、16、24、30 和 60 周龄时进行了采样。使用靶向 AbsoluteIDQ p180 试剂盒进行血浆代谢物谱分析，而 MI 酶试剂盒和 ELISA 分别用于定量组织 MI 浓度和 MI 关键酶（IMPase 1 和 MIOX）。作为主要发现，肾脏 MIOX 在 LB 和 LSL 母鸡中的表达不同，LB 中的含量更高。产蛋寿命从第 16 周到第 24 周的开始与代谢物谱的明显变化相关，但是 LSL 母鸡和 LB 母鸡的适应方式不同。在所有时间点对所有母鸡进行的 Pearson 相关性分析表明，MI 降解酶 MIOX 的高表达与代谢应激标志物有关。
