Department of Pathology, the First Affiliated Hospital of Fujian Medical University, Fuzhou, China.
Department of Pathology, Binhai Campus of the First Affiliated Hospital, National Regional Medical Center, Fujian Medical University, Fuzhou, 350212, China.
Sci Rep. 2024 Nov 3;14(1):26527. doi: 10.1038/s41598-024-78187-8.
To investigate the diagnostic value of intraoperative cytology and rapid immunocytochemistry in primary central nervous system lymphoma. 254 cases of lymphoma and 82 cases of non-lymphoma were collected from 2010 to 2023. Frozen section(FS) was using alone in 44 cases during 2010-2014, FS and intraoperative cytology(IC) were using in 251 cases during 2015 to 2022. Rapid immunocytochemical(RICC, CD20, GFAP) were using with FS + IC in 41 cases during 2021 to 2023. Method One: According to the results of archives, statistic the diagnostic accuracy of lymphoma during three time periods. Method Two: All cases were randomly renumbered, 4 neuropathologists compared the accuracy of independent histology and that of combining cytology. The archives showed the diagnostic accuracy of FS in PCNSL was 77.27%, FS + IC was 86.06%, FS + IC + RICC was 92.68%. The retrospective study demonstrated the diagnostic accuracy of FS was 79.76%, FS + IC was 87.33% and FS + IC + RICC was 92.68%. The positive predictive value, negative predictive value, sensitivity, specificity and accuracy of CD20 were 100%, 76.92%, 90.32%, 100% and 92.68%, respectively. The results of the paired χ test was no statistically significant difference (0.05 < P < 0.1) between FS + IC + RICC and immunohistochemical (IHC) diagnosis of paraffin sections. The integration of IC + RICC + FS diagnosis can significantly enhance the intraoperative diagnostic accuracy of PCNSL and rectify potential errors that may occurred when relying solely on FS diagnosis.
探讨原发性中枢神经系统淋巴瘤术中细胞学和快速免疫细胞化学的诊断价值。2010 年至 2023 年共收集淋巴瘤 254 例，非淋巴瘤 82 例。2010-2014 年单独使用冰冻切片（FS）44 例，2015-2022 年 FS 和术中细胞学（IC）联合使用 251 例。2021-2023 年 FS+IC 联合快速免疫细胞化学（RICC、CD20、GFAP）检查 41 例。方法一：根据档案结果，统计三个时期淋巴瘤的诊断准确率。方法二：将所有病例随机编号，4 位神经病理学家比较独立组织学和联合细胞学的准确率。档案显示 FS 诊断 PCNSL 的准确率为 77.27%，FS+IC 为 86.06%，FS+IC+RICC 为 92.68%。回顾性研究显示 FS 的诊断准确率为 79.76%，FS+IC 为 87.33%，FS+IC+RICC 为 92.68%。CD20 的阳性预测值、阴性预测值、敏感度、特异度和准确率分别为 100%、76.92%、90.32%、100%和 92.68%。配对 χ2 检验结果显示，FS+IC+RICC 与石蜡切片免疫组化（IHC）诊断无统计学差异（0.05<P<0.1）。IC+RICC+FS 联合诊断可显著提高 PCNSL 的术中诊断准确率，纠正单纯 FS 诊断可能出现的潜在误差。
