Centre for Urban Environmental Remediation, Beijing University of Civil Engineering and Architecture, Beijing 100044, China; Collaborative Innovation Center of Energy Conservation & Emission Reduction and Sustainable Urban-Rural Development in Beijing, Beijing University of Civil Engineering and Architecture, Beijing 100044, China.
Centre for Urban Environmental Remediation, Beijing University of Civil Engineering and Architecture, Beijing 100044, China; Collaborative Innovation Center of Energy Conservation & Emission Reduction and Sustainable Urban-Rural Development in Beijing, Beijing University of Civil Engineering and Architecture, Beijing 100044, China.
Bioresour Technol. 2025 Jan;416:131741. doi: 10.1016/j.biortech.2024.131741. Epub 2024 Nov 2.
In this study, hybrid constructed wetlands (HCW) with functional substrates (vermiculite-tourmaline modified polyurethane) were constructed to investigate nitrogen removal efficiency and metabolic cooperation mechanisms for treating rural contaminated surface water with natural temperature fluctuations. The results show that within a natural temperature fluctuation range of 9-25 °C, the HCW achieved an average nitrate nitrogen removal efficiency of 98 % and a total nitrogen removal efficiency of 76 %, with effluent total nitrogen less than 5 mg/L. The rational secretion of extracellular polymeric substance and the analysis of microbial community structure revealed that functional substrate favors biofilm formation, increases the activity of Candidatus_Brocadia and Thauera, and enhances ammonia and nitrate reduction. These findings elucidate the ecological patterns exhibited by microorganisms during the process of functional substrate intensification. Overall, this study offers valuable guidance for constructing HCW to treat contaminated surface water.
本研究构建了具有功能基质（蛭石-电气石改性聚氨酯）的复合湿地（HCW），以研究在自然温度波动条件下，用其处理农村受污染地表水的脱氮效率和代谢协同机制。结果表明，在 9-25℃的自然温度波动范围内，HCW 的硝酸盐氮去除率平均达到 98%，总氮去除率达到 76%，出水总氮小于 5mg/L。合理分泌胞外聚合物和微生物群落结构分析表明，功能基质有利于生物膜的形成，增加了 Candidatus_Brocadia 和 Thauera 的活性，增强了氨氮和硝酸盐氮的还原。这些发现阐明了功能基质强化过程中微生物的生态模式。总的来说，本研究为构建 HCW 处理受污染地表水提供了有价值的指导。
