Li Kehan, Wang Shengjie, Xu Chenyue, Ni Zhengyi, Wang Xiurong, Wang Fei
Department of Orthopaedic Surgery, Third Hospital of Hebei Medical University, Shijiazhuang, Hebei, China.
Department of Orthopaedic Surgery, Harrison International Peace Hospital, Hengshui, Hebei, China.
Comput Methods Biomech Biomed Engin. 2024 Nov 4:1-15. doi: 10.1080/10255842.2024.2422911.
The 5-methylcytosine (m5C) is a common post-transcriptional RNA methylation modification and is involved in the pathological process of many diseases. However, little is known about the role of m5C in osteoarthritis (OA). OA gene data and the corresponding information were downloaded from the Gene Expression Omnibus database. Based on 36 m5C regulators, we constructed the landscape and diagnostic model for OA. Later, two m5C modification patterns were identified, and functional analyses were performed to evaluate whether these patterns were related to endoplasmic reticulum (ER) stress and mitochondrial autophagy. We further comprehensively analyzed the immune cell infiltration characteristics in different modification patterns in OA. We also established the post-transcriptional regulatory networks and drug-gene networks. Our findings suggested that m5C regulators were differentially expressed between OA and normal samples and could serve as novel biomarkers for the diagnosis of OA. Besides, m5C regulators may be involved in regulating ER stress, mitochondrial autophagy, and immune infiltration in OA. The m5C modification can influence the sensitivity to drugs and the potential post-transcriptional regulatory mechanisms might provide promising targets.
5-甲基胞嘧啶（m5C）是一种常见的转录后RNA甲基化修饰，参与多种疾病的病理过程。然而，关于m5C在骨关节炎（OA）中的作用知之甚少。OA基因数据及相应信息从基因表达综合数据库下载。基于36个m5C调节因子，我们构建了OA的图谱和诊断模型。随后，鉴定出两种m5C修饰模式，并进行功能分析以评估这些模式是否与内质网（ER）应激和线粒体自噬相关。我们进一步全面分析了OA中不同修饰模式下的免疫细胞浸润特征。我们还建立了转录后调控网络和药物-基因网络。我们的研究结果表明，m5C调节因子在OA和正常样本之间存在差异表达，可作为OA诊断的新型生物标志物。此外，m5C调节因子可能参与调节OA中的ER应激、线粒体自噬和免疫浸润。m5C修饰可影响药物敏感性，潜在的转录后调控机制可能提供有前景的靶点。
