Choudhary Ratan Kumar, Choudhary Shanti, Tripathi Abhishek
College of Animal Biotechnology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana 141004, Punjab, India.
Hilltop Animal Hospital, Palatine, IL 60074, United States.
World J Stem Cells. 2024 Oct 26;16(10):896-899. doi: 10.4252/wjsc.v16.i10.896.
Recently, we read a mini-review published by Jeyaraman . The article explored the optimal methods for isolating mesenchymal stromal cells from adipose tissue-derived stromal vascular fraction (SVF). Key factors include tissue source, processing techniques, cell viability assessment, and the advantages/disadvantages of autologous allogeneic use. The authors emphasized the need for standardized protocols for SVF isolation, ethical and regulatory standards for cell-based therapy, and safety to advance mesenchymal stromal cell-based therapies in human patients. This manuscript shares our perspective on SVF isolation in canines. We discussed future directions to potentiate effective regenerative medicine therapeutics in human and veterinary medicine.
最近，我们阅读了杰亚拉曼发表的一篇小型综述。该文章探讨了从脂肪组织来源的基质血管成分（SVF）中分离间充质基质细胞的最佳方法。关键因素包括组织来源、处理技术、细胞活力评估以及自体/异体使用的优缺点。作者强调了制定SVF分离标准化方案、基于细胞疗法的伦理和监管标准以及安全性的必要性，以推进针对人类患者的基于间充质基质细胞的疗法。本手稿分享了我们对犬类SVF分离的观点。我们讨论了在人类和兽医学中增强有效再生医学治疗方法的未来方向。
