Denkhaus W, von Steldern D, Botzenhardt U, Konietzko H
Int Arch Occup Environ Health. 1986;57(2):109-15. doi: 10.1007/BF00381378.
To estimate the cellular immune response of workers highly exposed to mixtures of organic solvents, subpopulations of peripheral blood lymphocytes (PBLs) were analyzed. For this, the PBLs of nine floorers (aged 25-58 years, exposure time 8-35 years) were subsequently labelled with monoclonal antibodies OKT4, OKT8, OKT11, anti-Leu 7 and anti-Leu 12. Analysis was made by a FACS IV cell sorter (Becton-Dickinson, USA). The control group consisted of matched pairs of healthy donors. In the exposed group we found a decrease in the OKT11 (all) T cell fraction, a decrease in the OKT4 helper cells, an increase in the anti-Leu 7 positive cells, mostly natural killer cells, an important increase in anti-Leu 12 labelled T cells, i.e., human B-lymphocytes, and no differences in the OKT8 suppressor cells. Total fluorescence intensity profiles between the exposed and the unexposed group did not differ, i.e., the marker density on the cell surfaces remained unchanged. Similar changes in lymphocyte subpopulations are found in states of immunodeficiency and immunogenetic forms of aplastic anemia, a disease whose etiological relationship may be due to long-term exposure to organic solvents.
为评估高度暴露于有机溶剂混合物的工人的细胞免疫反应，对外周血淋巴细胞（PBL）亚群进行了分析。为此，随后用单克隆抗体OKT4、OKT8、OKT11、抗Leu 7和抗Leu 12标记了9名地板工人（年龄25 - 58岁，暴露时间8 - 35年）的PBL。通过FACS IV细胞分选仪（美国贝克顿 - 迪金森公司）进行分析。对照组由配对的健康供体组成。在暴露组中，我们发现OKT11（全部）T细胞亚群减少，OKT4辅助性细胞减少，抗Leu 7阳性细胞增加，主要是自然杀伤细胞，抗Leu 12标记的T细胞即人类B淋巴细胞显著增加，而OKT8抑制性细胞无差异。暴露组和未暴露组之间的总荧光强度分布没有差异，即细胞表面的标记密度保持不变。在免疫缺陷状态和再生障碍性贫血的免疫遗传形式中也发现了淋巴细胞亚群的类似变化，再生障碍性贫血这种疾病的病因关系可能归因于长期接触有机溶剂。
