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INTRODUCTION

In "Do-It-Yourself" (DIY) stores, workers from the wood department are considered woodworkers. Given the health risks associated with woodworking, particularly from fungi and their metabolites, this study aims to assess microbial contamination and health risks for both workers and customers.

METHODS

The study was developed in 13 DIY stores in Lisbon Metropolitan Area, Portugal. It employed a comprehensive sampling approach combining active (MAS-100, Andersen six-stage, Coriolis , and SKC Button Aerosol Sampler) and passive (electrostatic dust collectors, surface swabs, e-cloths, settled dust, filters from vacuumed dust, filtering respiratory protection devices, and mechanical protection gloves) methods to assess microbial contamination. A Lighthouse Handheld Particle Counter HH3016- IAQ was used to monitor the particulate matter size, temperature, and humidity.

RESULTS

The wood exhibition area presented the highest fungal load, while the payment area exhibited the highest bacterial load. MAS-100 detected the highest fungal load, and surface swabs had the highest bacterial load. sp. was the most frequently observed fungal species, followed by sp. Mycotoxins, namely mycophenolic acid, griseofulvin, and aflatoxin G1, were detected in settled dust samples and one filter from the vacuum cleaner from the wood exhibition area. Cytotoxicity evaluation indicates the wood-cutting area has the highest cytotoxic potential. Correlation analysis highlights relationships between fungal contamination and particle size and biodiversity differences among sampling methods.

DISCUSSION

The comprehensive approach applied, integrating numerous sampling methods and laboratory assays, facilitated a thorough holistic analysis of this specific environment, enabling Occupational and Public Health Services to prioritize interventions for accurate exposure assessment and detailed risk management.

INTRODUCTION

METHODS

RESULTS

DISCUSSION

简介

在“自己动手”（DIY）商店中，木材部门的工人被视为木工。鉴于与木工相关的健康风险，特别是来自真菌及其代谢物的风险，本研究旨在评估微生物污染对工人和顾客的健康风险。

方法

本研究在葡萄牙里斯本大都市区的 13 家 DIY 商店进行。它采用了综合采样方法，结合了主动（MAS-100、Andersen 六阶段、Coriolis 和 SKC 按钮气溶胶采样器）和被动（静电除尘器、表面拭子、e 布、沉降灰尘、从吸尘灰尘中过滤的过滤器、过滤呼吸防护设备和机械保护手套）方法来评估微生物污染。Lighthouse 手持式粒子计数器 HH3016-IAQ 用于监测颗粒物大小、温度和湿度。

结果

木材展示区的真菌负荷最高，而付款区的细菌负荷最高。MAS-100 检测到的真菌负荷最高，表面拭子的细菌负荷最高。 sp.是最常观察到的真菌物种，其次是 sp. 。在沉降灰尘样本和一个来自木材展示区的吸尘器过滤器中检测到真菌毒素，包括麦角酸、灰黄霉素和黄曲霉毒素 G1。细胞毒性评估表明，木材切割区具有最高的细胞毒性潜力。相关性分析突出了真菌污染与粒径之间的关系以及采样方法之间的生物多样性差异。