Yang Sheng-Yuan, Lin Yu-Ying, Hao Zheng, Li Zi-Jie, Peng Zheng-Qiang, Jin Tao
National Key Laboratory of Green Pesticide, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Center for R&D of Fine Chemicals of Guizhou University, Guiyang 550025, China.
Key Laboratory of Monitoring and Control of Tropical Agricultural and Forest Invasive Alien Pests of Ministry of Agriculture and Rural Affairs, Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China.
J Econ Entomol. 2024 Dec 28;117(6):2314-2327. doi: 10.1093/jee/toae234.
The endoparasitoid Asecodes hispinarum (Bouček) (Hymenoptera: Eulophidae) serves as an effective biological control agent against Brontispa longissima (Gestro) (Coleoptera: Chrysomelidae), a notorious palm pest. Endosymbionts found in parasitoids and their hosts have attracted significant attention due to their substantial influence on biocontrol efficacy. In this study, we employed 16S rRNA sequencing, polymerase chain reaction, and fluorescence in situ hybridization to assess the symbiotic bacteria composition, diversity, phylogeny, and localization in A. hispinarum and its host B. longissima. Our findings showed significant differences in the richness, diversity, and composition of symbiotic bacteria among different life stages of B. longissima. Notably, the bacterial richness, diversity, and composition of A. hispinarum was similar to that of B. longissima. Firmicutes and Proteobacteria were the dominant phyla, while Wolbachia was the dominant genera across the parasitoid and host. It was discovered for the first time that Wolbachia was present in A. hispinarum with a high infection rate at ≥ 96.67%. Notably, the Wolbachia strain in A. hispinarum was placed in supergroup A, whereas it was categorized under supergroup B in B. longissima. Furthermore, Wolbachia is concentrated in the abdomen of A. hispinarum, with particularly high levels observed in the ovipositors of female adults. These findings highlight the composition and diversity of symbiotic bacteria in both A. hispinarum and its host B. longissima, providing a foundation for the development of population regulation strategies targeting B. longissima.
内寄生蜂椰心叶甲啮小蜂（Asecodes hispinarum (Bouček)）（膜翅目：姬小蜂科）是防治椰心叶甲（Brontispa longissima (Gestro)）（鞘翅目：叶甲科）的一种有效生物防治剂，椰心叶甲是一种臭名昭著的棕榈害虫。由于寄生蜂及其寄主中的内共生菌对生物防治效果有重大影响，因此受到了广泛关注。在本研究中，我们采用16S rRNA测序、聚合酶链反应和荧光原位杂交技术，评估了椰心叶甲啮小蜂及其寄主椰心叶甲体内共生细菌的组成、多样性、系统发育和定位。我们的研究结果表明，椰心叶甲不同生命阶段的共生细菌丰富度、多样性和组成存在显著差异。值得注意的是，椰心叶甲啮小蜂的细菌丰富度、多样性和组成与椰心叶甲相似。厚壁菌门和变形菌门是主要的菌门，而沃尔巴克氏体是寄生蜂和寄主中的主要属。首次发现椰心叶甲啮小蜂体内存在沃尔巴克氏体，感染率高达≥96.67%。值得注意的是，椰心叶甲啮小蜂中的沃尔巴克氏体菌株属于A超群，而在椰心叶甲中则属于B超群。此外，沃尔巴克氏体集中在椰心叶甲啮小蜂的腹部，在雌性成虫的产卵器中含量特别高。这些发现突出了椰心叶甲啮小蜂及其寄主椰心叶甲体内共生细菌的组成和多样性，为制定针对椰心叶甲的种群调控策略提供了基础。
