College of Food and Bioengineering, Henan University of Science and Technology, Luoyang, P.R. China.
Department of Research and Development, Henan Animic Biotechnology Co., Ltd, Henan, China.
Sci Rep. 2024 Nov 4;14(1):26602. doi: 10.1038/s41598-024-78202-y.
This study aimed to evaluate the safety profile and beneficial effects of Heyndrickxia coagulans strain BC99 (BC99) for potential use in functional foods and pharmaceuticals. We began with whole genome sequencing of BC99, followed by a comprehensive safety assessment comprising genome analysis, hemolysis, cytotoxicity, antibiotic susceptibility tests, and cell adhesion and tolerance studies, along with acute and subacute oral toxicity studies in animal models. BC99 was isolated from a well-characterized collection originating from the feces of a healthy infant. Our results indicated no hemolytic activity on Columbia blood agar plates and broad antibiotic sensitivity, including to gentamicin, ampicillin, chloramphenicol, ciprofloxacin, and others. Cytotoxicity testing confirmed no adverse effects on HT-29 cells and significant adhesive properties to intestinal epithelial cells. Tolerance tests demonstrated over 90% viability of BC99 under simulated gastrointestinal conditions. In vivo studies in mice and rats confirmed the absence of adverse effects following oral administration. Collectively, these findings support BC99's robust tolerance to gastrointestinal environments, strong adhesion capabilities, and a broad spectrum of antibiotic resistance, underlining its potential as a safe and effective agent for gut microbiota modulation and host health enhancement.
本研究旨在评估海氏肠球菌 BC99 菌株（BC99）在功能性食品和药物中的安全性和有益效果。我们首先对 BC99 进行了全基因组测序，然后进行了全面的安全性评估，包括基因组分析、溶血、细胞毒性、抗生素敏感性试验、细胞黏附和耐受性研究，以及动物模型的急性和亚急性口服毒性研究。BC99 是从一个特征明确的粪便来源的健康婴儿的收集物中分离出来的。我们的结果表明，在哥伦比亚血琼脂平板上没有溶血活性，并且对包括庆大霉素、氨苄西林、氯霉素、环丙沙星等在内的多种抗生素具有广泛的敏感性。细胞毒性试验证实对 HT-29 细胞没有不良影响，并且对肠道上皮细胞具有显著的黏附特性。耐受力试验表明，BC99 在模拟胃肠道条件下的存活率超过 90%。在小鼠和大鼠的体内研究中，口服给予 BC99 后均未观察到不良反应。总的来说，这些发现支持 BC99 对胃肠道环境具有强大的耐受力、强烈的黏附能力和广谱的抗生素耐药性，强调了其作为调节肠道微生物群和增强宿主健康的安全有效药物的潜力。
